Development of N‐methyl‐D‐aspartate receptor subunit immunoreactivity in the neonatal gerbil cochlear nucleus
Development of N‐methyl‐D‐aspartate receptor subunit immunoreactivity in the neonatal gerbil cochlear nucleus
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DOI：
10.1002/(sici)1097-0029(19980501)41:3
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发表时间：
1998-05
影响因子：
2.5
通讯作者：
D. Joelson;I. Schwartz
中科院分区：
文献类型：
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作者：
D. Joelson;I. Schwartz
在出生后第7天（P），P14，P21和P28沙土鼠的耳蜗核中绘制了离子型N-甲基-D-天冬氨酸（NMDA）受体亚基的免疫反应性分布。将组织的冷冻切片和连续塑料切片与针对NMDAR 1（NR 1）、NMDAR 2A（NR 2A）、NMDAR 2A/B（NR 2A/B）和NMDAR 2 B（NR 2 B）的抗体一起孵育。在P7时观察到NR 1和NR 2A/B的总体弥漫性染色。从P7到P28，耳蜗背核分子层和梭形细胞层、耳蜗后腹核章鱼细胞区和耳蜗前腹核的神经元胞体染色增加。耳蜗背核和后腹侧核的神经元（未解决的质量的过程和轴突，不包括只有神经元胞体和明显染色的近端树突）的染色显示出稳定的下降，而分子层神经元保持中度染色。NR 2A抗体在耳蜗背核深层和梭形细胞层中产生独特的树突染色，首先在P14看到，在P21和P28染色的树突长度增加。耳蜗背核巨神经元是NR 2A染色最明显的胞体。染色的树突跨越耳蜗背核深部和梭形细胞层，甚至延伸到耳蜗后腹核的章鱼细胞区。树突状染色也存在于尾侧和嘴侧后腹侧耳蜗核，第一次可区分的P14，并变得越来越强。Chemicon多克隆NR 2B抗体产生的神经胶质染色在尾侧后腹侧耳蜗核和背侧耳蜗核梭形细胞层中尤其突出，在P7时最强烈，随后在P28的所有区域中减少，尽管没有消失。Molecular Probes（尤金，OR）多克隆NR 2 B在许多胞体上产生浅颗粒染色模式，但无神经胶质染色。用任一种NR 2B抗体，神经胶质细胞染色不显著。新生儿免疫反应模式的变化在不同人群的耳蜗核神经元胞体和树突的NR 1，NR 2 A，NR 2 A/B，和NR 2 B的差异表明，在一些受体组成的变化发生在整个听力发作的时期。Microsc. Res. Tech. 41：246-262，1998.© 1998 Wiley利斯公司
The distribution of immunoreactivity for the ionotropic N‐methyl‐D‐aspartate (NMDA) receptor subunits was mapped in the cochlear nucleus of postnatal day (P) 7, P14, P21, and P28 gerbils. Frozen sections and serial plastic sections of tissue were incubated with antibodies to NMDAR1 (NR1), NMDAR2A (NR2A), NMDAR2A/B (NR2A/B), and NMDAR2B (NR2B). An overall diffuse stain was noted at P7 for NR1 and NR2A/B. Staining of neuronal somata in the dorsal cochlear nucleus molecular layer and fusiform cell layer, the posteroventral cochlear nucleus octopus cell area, and the anteroventral cochlear nucleus increased from P7 to P28. Staining of the neuropil (the unresolved mass of processes and axons, excluding only neuronal somata and distinctly stained proximal dendrites) of the deep dorsal cochlear nucleus and posteroventral cochlear nucleus showed a steady decrease, while molecular layer neuropil remained moderately stained. The NR2A antibody produced a distinctive staining of dendrites in the dorsal cochlear nucleus deep and fusiform cell layers seen first at P14 with increasing dendritic lengths stained at P21 and P28. Giant neurons of the deep dorsal cochlear nucleus were the most conspicuous somata stained by the NR2A. Their stained dendrites spanned much of the dorsal cochlear nucleus deep and fusiform cell layers and even extended into the octopus cell area of the posteroventral cochlear nucleus. Dendritic staining was also present in caudal and rostral posteroventral cochlear nucleus, first distinguishable at P14 and becoming increasingly strong. The Chemicon polyclonal NR2B antibody produced glial staining especially prominent in the caudal posteroventral cochlear nucleus and the dorsal cochlear nucleus fusiform cell layer, most intense at P7 and subsequently decreasing, although not disappearing, in all areas through P28. The Molecular Probes (Eugene, OR) polyclonal NR2B produced a light granular staining pattern over a number of somata but no glial staining. Neuropil staining was not prominent with either NR2B antibody. Differences in changes of neonatal immunoreactivity patterns in different populations of cochlear nucleus neuronal somata and dendrites for NR1, NR2A, NR2A/B, and NR2B suggest that alterations in some receptor composition is occurring over the period spanning the onset of hearing. Microsc. Res. Tech. 41:246–262, 1998. © 1998 Wiley‐Liss, Inc.