Pharmacological characterization of dopamine receptors in the rice striped stem borer, Chilo suppressalis
Pharmacological characterization of dopamine receptors in the rice striped stem borer, Chilo suppressalis
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水稻二化螟多巴胺受体的药理学特征
DOI：
10.1016/j.ibmb.2017.03.004
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发表时间：
2017
影响因子：
3.8
通讯作者：
Huang Jia
中科院分区：
文献类型：
--
作者：
Xu Gang;Wu Shun-Fan;Gu Gui-Xiang;Teng Zi-Wen;Ye Gong-Yin;Huang Jia
多巴胺是脊椎动物和无脊椎动物中一种重要的神经递质和神经调质，也是昆虫中枢神经系统中含量最丰富的单胺。功能不同的多巴胺受体的补体通过改变细胞内Ca 2+和cAMP水平介导多巴胺的信号转导。本研究对水稻二化螟（Chilo suppressalis）的三种多巴胺受体CsDOP 1、CsDOP 2和CsDOP 3进行了初步鉴定。所有这三种受体都显示出与邻位多巴胺受体相当大的序列同一性。系统发育分析还将受体聚类在其各自的组内。CsDOP 1、CsDOP 2和CsDOP 3的转录水平在中枢神经系统中均高表达，表明它们在神经过程中具有重要作用。在HEK 293细胞中异源表达后，CsDOP 1、CsDOP 2和CsDOP 3被多巴胺和合成多巴胺受体激动剂剂量依赖性地激活。它们也可以被不同系列的拮抗剂阻断。本研究为进一步研究二化螟3种多巴胺受体在行为和生理上的作用提供了重要信息，并为开发新型杀虫剂提供了基础。
Dopamine is an important neurotransmitter and neuromodulator in both vertebrates and invertebrates and is the most abundant monoamine present in the central nervous system of insects. A complement of functionally distinct dopamine receptors mediate the signal transduction of dopamine by modifying intracellular Ca2+and cAMP levels. In the present study, we pharmacologically characterized three types of dopamine receptors, CsDOP1, CsDOP2 and CsDOP3, from the rice striped stem borer,Chilo suppressalis. All three receptors show considerable sequence identity with orthologous dopamine receptors. The phylogenetic analysis also clusters the receptors within their respective groups. Transcript levels ofCsDOP1,CsDOP2andCsDOP3were all expressed at high levels in the central nervous system, indicating their important roles in neural processes. After heterologous expression in HEK 293 cells, CsDOP1, CsDOP2 and CsDOP3 were dose-dependently activated by dopamine and synthetic dopamine receptor agonists. They can also be blocked by different series of antagonists. This study offers important information on three dopamine receptors fromC. suppressalisthat will provide the basis for forthcoming studies investigating their roles in behaviors and physiology, and facilitate the development of new insecticides for pest control.