Fibronectin adsorption on carbonate-containing hydroxyapatite
Fibronectin adsorption on carbonate-containing hydroxyapatite
复制标题
纤连蛋白在含碳酸盐羟基磷灰石上的吸附
DOI：
10.1016/j.ceramint.2021.01.017
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发表时间：
2021
影响因子：
5.2
通讯作者：
Kawashita Masakazu
中科院分区：
文献类型：
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作者：
Wang Yunting;Tsuru Kanji;Ishikawa Kunio;Yokoi Taishi;Kawashita Masakazu
含碳酸根的羟基磷灰石（CO 3-HAp）比纯羟基磷灰石具有更高的骨传导性，但其潜在机制尚不清楚。由于蛋白质在CO 3-HAp上的特异性吸附可能会影响细胞反应和骨传导性，我们研究了蛋白质吸附对CO 3-HAp观察到的高骨传导性的贡献。我们制备了不同碳酸盐含量（0.3，4.8，或8.3wt%）的CO 3-HAp粉末，并研究了纤维连接蛋白（Fn）在粉末上的吸附行为。结果表明，CO 3-HAp对Fn的吸附量随碳酸盐含量的增加而降低。尽管Hill或Langmuir等温线最适合于Fn在碳酸盐含量为0.3或4.8wt%的CO 3-HAp上的吸附，但Brunauer-Emmett-Teller等温线最适合于Fn在碳酸盐含量为8.3wt%的CO 3-HAp上的吸附。这些结果表明，CO 3-HAp的碳酸根含量影响Fn的吸附行为，并表明其特异性吸附有助于CO 3-HAp的高骨传导性。这些发现并不直接影响CO 3-HAp的临床应用，但它们可能导致开发具有高骨传导性的骨替代物。
Carbonate-containing hydroxyapatite (CO3-HAp) shows higher osteoconductivity than pure hydroxyapatite but the underlying mechanism is unclear. As specific adsorption of proteins on CO3-HAp may affect cellular responses and osteoconductivity, we examined the contribution of protein adsorption to the high osteoconductivity observed with CO3-HAp. We prepared CO3-HAp powders with different carbonate contents (0.3, 4.8, or 8.3 wt%) and investigated the adsorption behaviour of fibronectin (Fn) on the powders. We found that the Fn adsorption capacity of CO3-HAp decreased with increasing carbonate content. Although the Hill or Langmuir isotherm was the best-fit for the adsorption of Fn onto CO3-HAp with a carbonate content of 0.3 or 4.8 wt%, the Brunauer–Emmett–Teller isotherm was the best-fit for Fn adsorption on CO3-HAp with an 8.3 wt% carbonate content. These results show that the carbonate content of CO3-HAp influences the adsorption behaviour of Fn and suggest that its specific adsorption contributes to the high osteoconductivity of CO3-HAp. The findings do not affect the clinical application of CO3-HAp directly, but they might lead to the development of bone substitutes with high osteoconductivity.