THE AXONAL GLYCOPROTEIN TAG-1 IS AN IMMUNOGLOBULIN SUPERFAMILY MEMBER WITH NEURITE OUTGROWTH PROMOTING ACTIVITY
THE AXONAL GLYCOPROTEIN TAG-1 IS AN IMMUNOGLOBULIN SUPERFAMILY MEMBER WITH NEURITE OUTGROWTH PROMOTING ACTIVITY
复制标题
DOI：
10.1016/0092-8674(90)90223-2
复制
发表时间：
1990-04-06
期刊：
影响因子：
64.5
通讯作者：
JESSELL, TM
中科院分区：
文献类型：
--
作者：
FURLEY, AJ;MORTON, SB;JESSELL, TM
在发育中的神经系统中轴突的寻路被认为是由在胚胎轴突和生长锥表面上表达的糖蛋白介导的。一种被认为在轴突生长中起作用的分子是TAG-1，一种在发育中的哺乳动物神经系统中的神经元亚群的表面上瞬时表达的135 kd糖蛋白。我们分离了编码大鼠TAG-1的全长cDNA克隆。TAG-1具有六个免疫球蛋白样结构域和四个纤连蛋白III型样重复序列，并且在结构上与发育中轴突上表达的其他免疫球蛋白样蛋白相似。TAG-1的基板上保持在体外的神经元延长长的神经突，这表明该蛋白质在体内轴突的初始生长和指导中起作用。TAG-1通过糖基磷脂酰肌醇连接锚定到神经元膜上，并且也从神经元释放，这表明TAG-1在释放到细胞外环境中时也起底物粘附分子的作用。
Pathfinding of axons in the developing nervous system is thought to be mediated by glycoproteins expressed on the surface of embryonic axons and growth cones. One molecule suggested to play a role in axonal growth is TAG-1, a 135 kd glycoprotein expressed transiently on the surface of subsets of neurons in the developing mammalian nervous system. We isolated a full-length cDNA clone encoding rat TAG-1. TAG-1 has six immunoglobulin-like domains and four fibronectin type III-like repeats and is structurally similar to other immunoglobulin-like proteins expressed on developing axons. Neurons maintained in vitro on a substrate of TAG-1 extend long neurites, suggesting that this protein plays a role in the initial growth and guidance of axons in vivo. TAG-1 is anchored to the neuronal membrane via a glycoslyl phosphatidylinositol linkage and is also released from neurons, suggesting that TAG-1 also functions as a substrate adhesion molecule when released into the extracellular environment.