Studies on sterol-ester hydrolase from Fusarium oxysporum. I. Partial purification and properties.
Studies on sterol-ester hydrolase from Fusarium oxysporum. I. Partial purification and properties.
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尖孢镰刀菌甾醇酯水解酶的研究。
DOI：
10.1093/oxfordjournals.jbchem.a131571
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发表时间：
1977
影响因子：
2.7
通讯作者：
T. Yamaguchi
中科院分区：
文献类型：
--
作者：
Y. Okawa;T. Yamaguchi
1. 在微生物中寻找一种长链脂肪酸酰基甾醇酯水解酶，从土壤中分离出镰刀菌属的5株菌株，这些菌株在培养基中产生了很强的活性。2. 采用CaCl2沉淀法和Sephadex G-75柱层析法对尖孢镰刀菌（Fusarium oxysporum） igh2中的胆固醇酯酶进行了270倍的纯化。3. 戊卡醇和Triton X-100激活胆固醇酯酶。它被卵磷脂和溶卵磷脂抑制，并被热处理（60℃30 min, pH 7.0）完全灭活。4. 酶的最适pH值为7.0左右。5. 在所测试的各种胆固醇酯中，亚油酸胆固醇是最合适的底物。6. 血清中的胆固醇酯也被这种酶水解。
1. A search for a long chain fatty acyl sterol-ester hydrolase in microorganisms led to the isolation from soil of five strains belonging to Fusarium sp. which produced strong activity in the culture medium. 2. The cholesterol esterase from Fusarium oxysporum IGH-2 was purified about 270-fold by means of CaCl2 precipitation and Sephadex G-75 column chromatography. 3. The cholesterol esterase was activated by adekatol and Triton X-100. It was inhibited by lecithin and lysolecithin, and completely inactivated by heat treatment (60 degrees C for 30 min, at pH 7.0). 4. The optimum pH of the enzyme was found to be around 7.0. 5. Among various cholesterol esters tested, cholesterol linoleate was the most suitable substrate. 6. Cholesterol esters in serum were also hydrolyzed by this enzyme.