Bacillomycin D and its combination with amphotericin B: promising antifungal compounds with powerful antibiofilm activity and wound-healing potency
Bacillomycin D and its combination with amphotericin B: promising antifungal compounds with powerful antibiofilm activity and wound-healing potency
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DOI：
10.1111/jam.13030
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发表时间：
2016-02-01
影响因子：
4
通讯作者：
Mangoni, M. L.
中科院分区：
文献类型：
--
作者：
Tabbene, O.;Azaiez, S.;Mangoni, M. L.
AimsIn this study，we evaluated the ability of the lipopeptide bacillomycin D and the anti真菌drug acettericin B as well as their combination，to inhibit Candida albicans biofilm formation and to accelerate keratinocyte cellmigration.Methods and ResultsThe bacillomycin D and its combination with acettericin B as well as their combination was carried out by crystal violet colorimetry method.我们的结果表明，当以对哺乳动物细胞无毒的低浓度组合在一起时，分别对应于芽孢杆菌霉素D和枯草杆菌素B的1/32 MIC（0.39 gml（-1））和1/4 MIC（0.06 gml（-1）），表现出明显的生物膜活性（生物膜形成的95%抑制）沿着明显的芽管形成抑制。此外，使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物（MTT）还原测定来测定两种药物对白色念珠菌菌株的预形成生物膜的影响。两种抗真菌化合物的组合，分别为0.39和1 μ g ml（-1）的芽孢杆菌霉素D和枯草杆菌素B，导致生物膜根除的明显增强，与单独使用每种药物获得的结果相比。此外，委员会认为，结论细菌霉素D及其与抗真菌药白曲霉素B的联合应用具有显著的抗生物膜和伤口愈合活性，其在医疗器械中的应用可能为念珠菌的局部治疗提供一种有前途的选择。在伤口处的相关感染。
AimsIn this study, we evaluated the ability of the lipopeptide bacillomycin D and the antifungal drug amphotericin B as well as their combination, to inhibit Candida albicans biofilm formation and to accelerate keratinocyte cell migration.Methods and ResultsThe antibiofilm activity of bacillomycin D and its combination with amphotericin B was carried out by crystal violet colorimetric method. Our results have shown that, when combined together at low concentrations nontoxic to mammalian cells, corresponding to 1/32 MIC (039gml(-1)) and 1/4 MIC (006gml(-1)) for bacillomycin D and amphotericin B, respectively, a clear antibiofilm activity is manifested (95% inhibition of biofilm formation) along with a clear inhibition of germ tube formation. Moreover, the effect of both drugs on preformed biofilm of C.albicans strain was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. The combination of the two antifungal compounds at 039 and 1gml(-1) for bacillomycin D and amphotericin B, respectively, resulted in a clear enhancement of biofilm eradication compared to the results obtained with each drug alone. Furthermore, this combination was found to promote the closure of a gap produced in a monolayer of human keratinocytes.ConclusionsBacillomycin D and its combination with amphotericin B display impressive anti-biofilm and wound-healing activities.Significance and Impact of the StudyApplication of the lipopeptide bacillomycin D and the antifungal drug amphotericin B in medical devices may offer a promising alternative for topical treatment of Candida-associated infections in the setting of a wound.