The transcriptomic profiles of adipose tissues are modified by feed deprivation in lactating goats
The transcriptomic profiles of adipose tissues are modified by feed deprivation in lactating goats
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DOI：
10.1016/j.cbd.2010.12.002
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发表时间：
2011-06-01
影响因子：
3
通讯作者：
Leroux, C.
中科院分区：
文献类型：
--
作者：
Faulconnier, Y.;Chilliard, Y.;Leroux, C.
反刍动物脂肪组织的主要功能是储存用于生产功能的脂质。在能量负平衡的时期，如哺乳期或营养不良，需要进行身体脂肪动员。到目前为止，反刍动物脂肪组织的基因表达谱响应营养限制尚未进行。为了更好地了解脂肪组织中的分子机制，在饮食因素的反应，微阵列分析被用来比较两个极端的营养条件（控制饮食与48小时的饲料剥夺）的影响，在网膜和肾周脂肪组织的泌乳山羊（山羊）。我们观察到456和199个基因在精神和肾周脂肪组织的表达改变，分别。在这两个网站的饲料剥夺改变了类似的生物过程，虽然有两倍的基因差异表达的网膜比肾周脂肪组织。两者合计，参与脂质代谢的转录变化（脂质合成和甘油三酯储存能力降低以及脂肪酸氧化增加）与山羊脂肪组织中响应于48小时禁食的能量沉积减少一致。48小时禁食后观察到脂肪组织的炎症状态。(C)2010年由Elsevier Inc.出版
A major function of ruminant adipose tissue is to store lipids for use in productive functions. Body fat mobilization is required during periods of negative energy balance such as lactation or undernutrition. Until now, gene expression profiling of ruminant adipose tissue in response to nutritional restriction has not been performed. To gain a better understanding of the molecular mechanisms in adipose tissue in response to dietary factors, microarray analysis was used to compare the effects of two extreme nutritional conditions (control diet vs. 48-h feed deprivation) in the omental and perirenal adipose tissues of lactating goats (Capra hircus). We observed the altered expression of 456 and 199 genes in mental and perirenal adipose tissues, respectively. Similar biological processes were altered by feed deprivation in these two sites, although twice as many genes were differentially expressed in the omental than in the perirenal adipose tissue. Taken together, the transcriptional changes involved in lipid metabolism (decreased lipid synthesis and triglyceride storage capacity as well as increased fatty acid oxidation) were consistent with reduced energy deposition in goat adipose tissues in response to a 48-h fast. An inflammatory state of the adipose tissue was observed following the 48-h fast. (C) 2010 Published by Elsevier Inc.