Differential expression of genes involved in the biosynthesis and perception of ethylene during ripening of passion fruit (Passiflora edulis sims)
Differential expression of genes involved in the biosynthesis and perception of ethylene during ripening of passion fruit (Passiflora edulis sims)
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DOI：
10.1093/oxfordjournals.pcp.a029322
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发表时间：
1998-11-01
影响因子：
4.9
通讯作者：
Hyodo, H
中科院分区：
文献类型：
--
作者：
Mita, S;Kawamura, S;Hyodo, H
1-氨基环丙烷-1-羧酸（ACC）合成酶和ACC氧化酶的酶活性的增加诱导乙烯的进化在西番莲果实成熟。在成熟过程中，假种皮中产生的乙烯含量比种子或果皮中高得多。两个ACC合酶基因（PE-ACS 1和PE-ACS 2），一个ACC氧化酶基因（PE-ACO 1），和两个乙烯受体基因（PE-ETR 1和PE-ERS 1）的表达模式显示，这些基因的表达差异调节。PE-ACS 1和PE-ACO 1的表达在成熟过程中和乙烯处理后增强。然而，与PE-AC 01相比，PE-A CSI的显著表达延迟。大量的PE-ACS 1 mRNA和PE-ACO 1 mRNA被认为是在假种皮比种子，这对应于增加乙烯产生的植物组织本身的量，PE-ACS 2 mRNA的水平是可检测到的preclimateric果实的假种皮，虽然它在成熟过程中下降。这些结果表明，PE-ACS 1和PE-ACO 1的表达是提高成熟过程中乙烯生物合成酶活性所必需的。PE-ETR 1和PE-ERS 1的表达水平在成熟过程中没有显著变化;然而，PE-ETR 1和PE-ERS 1的mRNA水平在假种皮中比在种子中高得多。
An increase in the enzyme activity of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase induces the evolution of ethylene during the ripening of passion fruit. A much higher level of ethylene is produced in arils than in seeds or peels during ripening. The pattern of expression of two ACC synthase genes (PE-ACS1 and PE-ACS2), one ACC oxidase gene (PE-ACO1), and two ethylene receptor genes (PE-ETR1 and PE-ERS1) revealed that the expression of these genes is differentially regulated. Expression of PE-ACS1 and PE-ACO1 was enhanced during ripening and after ethylene treatment. However, prominent expression of PE-A CSI was delayed compared to that of PE-ACO1. Much larger quantities of PE-ACS1 mRNA and PE-ACO1 mRNA were seen in arils than in seeds; this corresponds well with an increase in the amount of ethylene produced by the plant tissue itself, The level of PE-ACS2 mRNA was detectable in arils of the preclimacteric fruit, although it decreased during ripening. These results suggest that expression of PE-ACS1 and PE-ACO1 is required to increase the activity of ethylene biosynthetic enzymes during ripening. The level of expression of PE-ETR1 and PE-ERS1 did not significantly change over the course of ripening; however, the mRNA levels of PE-ETR1 and PE-ERS1 were much higher in arils than in seeds.