Control of fusion pore dynamics during exocytosis by Munc18
Control of fusion pore dynamics during exocytosis by Munc18
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DOI：
10.1126/science.291.5505.875
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发表时间：
2001-02-02
期刊：
影响因子：
56.9
通讯作者：
Burgoyne, RD
中科院分区：
文献类型：
--
作者：
Fisher, RJ;Pevsner, J;Burgoyne, RD
细胞内膜融合由 SNARE（可溶性 N-乙基马来酰亚胺敏感因子附着蛋白受体）蛋白介导。所有囊泡转运步骤也对 Sec1 蛋白家族成员有一个基本要求，包括调节胞吐作用中的神经元 Munc18-1（也称为 nSec1）。在这里，在肾上腺嗜铬细胞中，我们表达了对突触蛋白亲和力降低的 Munc18 突变体，该突变体特异性地改变了单颗粒胞吐释放事件的动力学，与融合孔扩张的加速一致。因此，Munc18 在融合过程的后期发挥作用，其中它与突触蛋白的解离决定了融合后事件的动力学。
Intracellular membrane fusion is mediated by the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins. All vesicle transport steps also have an essential requirement for a member of the Sec1 protein family, including the neuronal Munc18-1 (also known as nSec1) in regulated exocytosis. Here, in adrenal chromaffin cells, we expressed a Munc18 mutant with reduced affinity for syntaxin, which specifically modified the kinetics of single-granule exocytotic release events, consistent with an acceleration of fusion pore expansion, Thus, Munc18 functions in a late stage in the fusion process, where its dissociation from syntaxin determines the kinetics of postfusion events.