NEUROPEPTIDE-Y AND BEHAVIORALLY INDUCED PHASE-SHIFTS
NEUROPEPTIDE-Y AND BEHAVIORALLY INDUCED PHASE-SHIFTS
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DOI：
10.1016/0306-4522(94)90331-x
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发表时间：
1994-09-01
期刊：
影响因子：
3.3
通讯作者：
MROSOVSKY, N
中科院分区：
文献类型：
--
作者：
BIELLO, SM;JANIK, D;MROSOVSKY, N
含神经肽Y的纤维从外侧膝状体核的膝状体间叶投射到视交叉上核。先前的研究表明，这一通路可能参与了生物钟的非光重置。因此，我们研究了神经肽Y介导由特定的非光刺激引起的相移的可能性，即在一个新的轮子中运行的脉冲。将仓鼠限制在一个小巢箱中未能阻止在zeitgeber时间4给予神经肽Y诱导的相移;这表明增加的运动活性对于所观察到的移位是不必要的。在昼夜节律的时间5通过插管针对视交叉上核的抗血清或正常血清。然后将仓鼠从它们的笼子中取出，并放置在新型轮中3小时。接受正常血清并在新型轮中运行> 5000转的仓鼠推进其节律（平均偏移2.55 h +/-0.22 S.E.M.）其数量与未手术的仓鼠相似。神经肽Y抗血清的给药减弱了通常与在新轮子中跑步相关的偏移（平均偏移0.21 +/- 0.14 S.E.M.）。这些研究表明，从外侧膝状体核输入到生物钟的神经肽Y参与了对新奇感诱导的车轮运行的反应中所见的相移。它也提供了抗血清改变行为能力的另一个例子。这可能是一个有用的方法，在操纵神经化学活性时，拮抗剂还没有或定义不清。
Neuropeptide Y-containing fibers project from the intergeniculate leaflet of the lateral geniculate nucleus to the suprachiasmatic nucleus. Previous studies have indicated that this pathway may be involved in non-photic resetting of the circadian clock. Therefore, we investigated the possibility that neuropeptide Y mediates phase shifts induced by a particular non-photic stimulus, a pulse of running in a novel wheel. Confining hamsters to a small nest box failed to block phase shifts induced by neuropeptide Y given at zeitgeber time 4; this indicates that increased locomotor activity is not necessary for the observed shifts. Antiserum raised against neuropeptide Y or normal serum was administered at circadian time 5 through a cannula aimed at the suprachiasmatic nucleus. The hamsters were then removed from their cages and placed in a novel wheel for 3 h. Hamsters that received normal serum and ran > 5000 revolutions in the novel wheel advanced their rhythms (mean shift 2.55 h +/- 0.22 S.E.M.) by amounts similar to those of unoperated hamsters. Administration of neuropeptide Y antiserum attenuated the shift normally associated with running in a novel wheel (mean shift 0.21 +/- 0.14 S.E.M.).These studies indicate that the neuropeptide Y input from the lateral geniculate nucleus to the biological clock is involved in the phase shifts seen in response to novelty-induced wheel running. It also provides another example of the ability of antisera to alter behavior. This may be a useful approach in manipulations of neurochemical activity when antagonists are not yet available or poorly defined.