METHYLATION OF REPLICATING AND POST-REPLICATED MOUSE L-CELL DNA
METHYLATION OF REPLICATING AND POST-REPLICATED MOUSE L-CELL DNA
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DOI：
10.1073/pnas.80.16.4919
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
RAZIN, A
中科院分区：
文献类型：
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作者：
GRUENBAUM, Y;SZYF, M;RAZIN, A
[α-32P]dGTP进入通透性细胞，并通过最近邻分析测量CpG位点的甲基化程度。此方法显示.apprx的滞后。DNA合成和修饰事件之间的1分钟。当加入S-腺苷-L-同型半胱氨酸在持续的脱氧核糖核酸合成的情况下抑制甲基化时，产生的半甲基化位点在抑制释放后立即甲基化。显然，细胞中的甲基酶活性允许立即甲基化，但复制分叉处的条件会导致修饰反应的开始短暂延迟。
[.alpha.-32P]dGTP into permeabilized cells and measured the degree of methylation at CpG sites by nearest-neighbor analysis. This method reveals a lag of .apprx. 1 min between DNA synthesis and the modification event. When methylation is inhibited by the addition of S-adenosyl-L-homocysteine in the presence of continued DNA synthesis, the resulting hemimethylated sites are methylated immediately after the release of inhibition. Evidently, the methylase activity in the cell allows immediate methylation, but conditions at the replication fork bring about a short delay in the onset of the modification reaction.