Dorsal telencephalon-specific expression of Cre recombinase in PAC transgenic mice
Dorsal telencephalon-specific expression of Cre recombinase in PAC transgenic mice
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DOI：
10.1002/gene.20009
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发表时间：
2004-03-01
期刊：
影响因子：
1.5
通讯作者：
Itohara, S
中科院分区：
文献类型：
--
作者：
Iwasato, T;Nomura, R;Itohara, S
将基因表达或破坏限制在大脑特定区域的能力将增强对大脑发育和功能的分子基础的理解。为此，脑区域限制性启动子是必不可少的。在这里，我们报告的分离的DNA片段包含Emx 1基因启动子，这是负责背端脑特异性表达。将Cre重组酶基因插入小鼠PAC（P1衍生的人工染色体）Emx 1基因座克隆（PAC-Emx 1#1克隆）中，并用于产生三个转基因小鼠系。在所有三个线，特别是Tg 3，Cre介导的重组是高度限制Emx 1表达细胞系，从胚胎期到成年期。免疫组织化学分析表明，Cre蛋白表达在背侧端脑在所有三个线在成年期。因此，PAC-Emx 1 #1克隆基本上含有Emx 1基因表达所需的所有调节元件。我们的研究结果表明，Emx 1-Cre Tg 3小鼠和PAC-Emx 1 #1克隆构成了背侧端脑特异性基因操作的强大工具。(C)2004 Wiley-Liss，Inc.
The ability to restrict gene expression or disruption to specific regions of the brain would enhance understanding of the molecular basis for brain development and function. For this purpose, brain region-restricted promoters are essential. Here we report the isolation of a DNA fragment containing the Emx1 gene promoter, which is responsible for dorsal telencephalon-specific expression. The Cre recombinase gene was inserted into a mouse PAC (P1-derived artificial chromosome) Emx1-locus clone (PAC-Emx1#1 clone) and utilized to generate three transgenic mouse lines. In all three lines, especially Tg3, Cre-mediated recombination was highly restricted to Emx1-expressing cell lineages, from embryonic stages to adulthood. Immuno-histochemical analyses showed that Cre protein is expressed in the dorsal telencephalon in all three lines in adulthood. Thus, the PAC-Emx1#1 clone contains essentially all regulatory elements necessary for Emx1 gene expression. Our results suggest that Emx1-Cre Tg3 mice and the PAC-Emx1#1 clone constitute powerful tools for dorsal telencephalon-specific gene manipulation. (C) 2004 Wiley-Liss, Inc.