3D Imaging of Lipid-Guided Vesicle Trafficking Along the Cytoskeleton
3D Imaging of Lipid-Guided Vesicle Trafficking Along the Cytoskeleton
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脂质引导的囊泡沿细胞骨架运输的 3D 成像
DOI：
10.1016/j.tplants.2020.12.019
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发表时间：
2021
影响因子：
20.5
通讯作者：
Jinxing Lin
中科院分区：
文献类型：
--
作者：
Jingjing Xing;Zhikun Duan;Liang Zhang;Xi Zhang;Jinxing Lin
在体内标记脂质是探索协调磷脂信号传导和膜运输的详细分子机制的关键步骤。利用脂质结合域与各种荧光蛋白（FP）[如单色FP，光活化FP和基于Förster共振能量转移（FRET）的FP]融合，脂质生物传感器用于监测目标脂质的时空分布，浓度和动态特性。
Labeling lipids in vivo is a crucial step in exploring detailed molecular mechanisms coordinating phospholipid signaling and membrane trafficking. Using a lipid-binding domain fused with various fluorescent proteins (FPs)[such as single-colored FP, photoactivated-FP and FPs based on Förster resonance energy transfer (FRET)], lipid biosensors are used to monitor the spatiotemporal distribution, concentration, and dynamic characteristics of targeted lipids.