THE ORGANIZATION OF PREFRONTOCAUDATE PROJECTIONS AND THEIR LAMINAR ORIGIN IN THE MACAQUE MONKEY - A RETROGRADE STUDY USING HRP-GEL
THE ORGANIZATION OF PREFRONTOCAUDATE PROJECTIONS AND THEIR LAMINAR ORIGIN IN THE MACAQUE MONKEY - A RETROGRADE STUDY USING HRP-GEL
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DOI：
10.1002/cne.902440407
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发表时间：
1986-02-22
影响因子：
2.5
通讯作者：
KUBOTA, K
中科院分区：
文献类型：
--
作者：
ARIKUNI, T;KUBOTA, K
用辣根过氧化物酶聚丙烯酰胺凝胶研究了猕猴Monjey的额尾状核前连接的组织结构，该凝胶在胼胝体切开术后注入尾状核的头部。用四甲基联苯胺处理组织以测定过氧化物酶活性。逆行标记细胞出现在双侧前额叶皮质，并在同侧的运动前区，额盖，颞叶和吻侧区的皮质。在前额叶皮层，标记细胞位于皮层2-6层。第2层偶见标记细胞。第3层可见大量标记细胞，主要分布在其下部。它们是锥体细胞。第4层含有相当数量的标记细胞，呈锥体或圆形。这些标记细胞的体细胞大小为5至15 μ m，但小于5 μ m的细胞均未被标记。第5层密集地填充有标记的细胞，尽管在其上部（5a）中密度更高。它们是锥体细胞。在第6层，锥体或梭形细胞标记的密度低于第5层。标记细胞的层状模式和密度在前额叶皮层的不同区域之间是不同的，但是，在一般情况下，颗粒上的标记往往经常发生在含有大量标记细胞的区域。含有大多数标记细胞的前额区与尾状核头部的注射部位之间存在地形关系：内侧眶额皮质和从喙沟向下延伸的内侧表面同侧地突出到核的头部的腹内侧部分的近内侧部分，外侧眶额皮质投射到核头部的腹内侧部分，主沟腹侧的背外侧前额叶皮质投射到核头部的中央部分。我们在猕猴的研究结果表明，皮质尾状核连接的传出神经元的层状组织的皮质皮质连接。
The organization of the prefrontocaudate connection in the macaque monjey was studied with a polyacrylamide gel of horseradish peroxidase that was injected, after callosotomy, into the head of the caudate nucleus. Tissue were processed for peroxidase activity with tetramethylbenzidine. Retrogradely labeled cells appeared bilaterally in the prefrontal cortex, and ipsilaterally in the premotor area, frontal operculum, insula, and rostral region of the temporal cortex. In the prefrontal cortex, labeled cells were located in cortical layers 2-6. In layer 2, labeled cells were occasionally seen. In layer 3, a considerable number of labeled cells were seen and were mainly distributed in its lower part. They were pyramidal cells. Layer 4 contained a considerable number of labeled cells that were pyramidal or round. Somal sizes of these labeled cells were from 5 to 15 .mu.m, but none of the cells less than 5 .mu.m were labeled. Layer 5 was densely packed with labeled cells, though at a higher density in its upper part (5a). They were pyramidal cells. In layer 6, pyramidal or fusiform cells were labeled at a lower density than in layer 5. The laminar pattern and density of labeled cells were different among various regions of the prefrontal cortex, but, in general, the supragranular labeling tended to frequently occur in the regions containing the numerous labeled cells. There was a topographical relationship between the prefrontal regions containing the majority of labeled cells and the injection sites in the head of the caudate nucleus: the medial orbitofrontal cortex and the medial surface extending downward from the rostral sulcus project ipsilaterally to the medialmost portion of the ventromedial part of the head of the nucleus, the lateral orbitofrontal cortex projects to the ventromedial part of the head of the nucleus, and the dorsolateral prefrontal cortex ventral to the principal sulcus projects to the central part of the head of the nucleus. Our findings in the macaque monkey indicate that the corticocaudate connection is similar in laminar organization of efferent neurons to the corticocortical connections.