Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae.
Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae.
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DOI：
10.1099/ijs.0.022186-0
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发表时间：
2011-03
影响因子：
2.8
通讯作者：
Yu-Qin Zhang;Hong-yu Liu;Li-Yan Yu;Jae-Chan Lee;DongJin Park;Chang-Jin Kim;Lihua Xu;Cheng-lin Jiang-Cheng-li
中科院分区：
文献类型：
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作者：
Yu-Qin Zhang;Hong-yu Liu;Li-Yan Yu;Jae-Chan Lee;DongJin Park;Chang-Jin Kim;Lihua Xu;Cheng-lin Jiang-Cheng-li
从中国西北部青海省收集的土壤样品中分离出一种革兰氏阳性、需氧、非运动性放线菌，命名为菌株 6014(T)，并进行了多相分类学研究。该分离株在 ISP 4 和 R2A 培养基上形成初级分枝菌丝和丰富的气生菌丝体，并具有球形孢子囊。菌株6014(T)的全细胞水解产物含有作为诊断糖的阿拉伯糖、半乳糖和核糖以及作为诊断二氨基酸的内消旋二氨基庚二酸。极性脂质由磷脂酰甲基乙醇胺、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、含N-乙酰葡糖胺的磷脂、两种未知磷脂和未知糖脂组成。甲基萘醌体系包含MK-9(H2)和MK-9(H4)。主要脂肪酸是C 14:0、i-C 15:0、C 16:0 和10-甲基-C 16:1。分离物的基因组DNA G+C含量为69.4 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株6014（T）属于链孢子菌亚目的半径范围内，该菌株在该亚目中形成了与链孢子科属相邻的独特谱系。根据这项多相研究的数据，菌株 6014(T) 可以很容易地与之前描述的生物体区分开来，并且代表链孢菌亚目新属内的新物种的成员。名称：Sinosporangium album gen.十一月，SP。十一月建议使用 6014(T) (=DSM 45181(T) =KCTC 19655(T)) 作为类型应变。
A Gram-positive, aerobic, non-motile actinobacterium, designated strain 6014(T), was isolated from a soil sample collected from Qinghai province, north-west China, and subjected to a polyphasic taxonomic study. The isolate formed elementary branching hyphae and abundant aerial mycelia with globose sporangia on ISP 4 and R2A media. Whole-cell hydrolysates of strain 6014(T) contained arabinose, galactose and ribose as diagnostic sugars and meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipids consisted of phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, two unknown phospholipids and an unknown glycolipid. The menaquinone system contained MK-9(H₂) and MK-9(H₄). The major fatty acids were C₁₄:₀, i-C₁₅:₀, C₁₆:₀ and 10-methyl-C₁₆:₁. The genomic DNA G+C content of the isolate was 69.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 6014(T) fell within the radius of the suborder Streptosporangineae, in which the strain formed a distinct lineage next to genera of the family Streptosporangiaceae. Based on data from this polyphasic study, strain 6014(T) can be readily distinguished from previously described organisms and represents a member of a novel species within a new genus in the suborder Streptosporangineae. The name Sinosporangium album gen. nov., sp. nov. is proposed with 6014(T) (=DSM 45181(T) =KCTC 19655(T)) as the type strain.