Regulation of the mitosis/meiosis decision in the Caenorhabditis elegans germline
Regulation of the mitosis/meiosis decision in the Caenorhabditis elegans germline
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DOI：
10.1098/rstb.2003.1333
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发表时间：
2003-08-29
期刊：
影响因子：
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通讯作者：
Kimble, J
中科院分区：
文献类型：
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作者：
Crittenden, SL;Eckmann, CR;Kimble, J
在多细胞生物体的发育过程中，生长和分化过程保持平衡，以产生和维持正确的大小、形状和细胞组成的组织和器官。我们研究了秀丽线虫种质系生长和分化的分子控制。一个单一的体细胞，称为远端顶端细胞，通过GLP-1/Notch信号促进邻近生殖系的有丝分裂增殖。在生殖系中，有丝分裂和减数分裂之间以及精子发生和卵子发生之间的决定由一组保守的RNA调节因子控制。171317是核糖核酸结合蛋白家族的成员，通过抑制GLD-1mRNA活性促进有丝分裂；GLD-1、GLD-2、GLD-3和NOS-3蛋白通过调节未知的mRNAs促进减数分裂。相反的FBF和GLD活性之间的调节平衡对于控制生殖系增殖的程度至关重要。PUF蛋白调节果蝇和线虫的生殖系干细胞，并定位于哺乳动物睾丸的生殖系干细胞。因此，这种转录后调控开关可能是控制干细胞维持与分化的古老机制。
During the development of multicellular organisms, the processes of growth and differentiation are kept in balance to generate and maintain tissues and organs of the correct size, shape and cellular composition. We have investigated the molecular controls of growth and differentiation in the Caenorhabditis elegans germline. A single somatic cell, called the distal tip cell, promotes mitotic proliferation in the adjacent germline by GLP-1/Notch signalling. Within the germline, the decisions between mitosis and meiosis and between spermatogenesis and oogenesis are controlled by a group of conserved RNA regulators. 171317, a member of the PUF (for Pumilio and FBF) family of RNA-binding proteins, promotes mitosis by repressing gld-1 mRNA activity; the GLD-1, GLD-2, GLD-3 and NOS-3 proteins promote entry into meiosis by regulating mRNAs that remain unknown. The regulatory balance between opposing FBF and GLD activities is crucial for controlling the extent of germline proliferation. PUF proteins regulate germline stem cells in both Drosophila and C. elegans and are localized to germline stem cells of the mammalian testis. Therefore, this post-transcriptional regulatory switch may be an ancient mechanism for controlling maintenance of stem cells versus differentiation.