Angiogenesis induced by controlled release of neuropeptide substance P
Angiogenesis induced by controlled release of neuropeptide substance P
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DOI：
10.1016/j.biomaterials.2010.07.079
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发表时间：
2010-11-01
期刊：
影响因子：
14
通讯作者：
Tabata, Yasuhiko
中科院分区：
文献类型：
--
作者：
Kohara, Hiroshi;Tajima, Shuhei;Tabata, Yasuhiko
循环宿主细胞在体内募集到待再生的位点是治疗性血管生成的有前景的策略之一。P物质（SP）是神经肽的一员，介导疼痛感知并调节伤口愈合、炎症、肿瘤细胞增殖和血管生成。该SP在体外增强粒细胞的迁移、粘附和血管生成基因表达。以明胶的阴离子衍生物为原料，制备了一种可生物降解的水凝胶，以实现SP在体内的控释。当将含有SP的阴离子明胶水凝胶皮下植入小鼠背部时，与注射SP溶液相反，在植入部位周围诱导了显著的血管生成。在植入部位周围观察到粒细胞的体内蓄积。它的结论是，控制释放的SP有效地诱导招聘和随后的激活粒细胞，一个循环细胞与血管生成活动，从血液循环到植入部位，导致增强血管生成。(C)2010爱思唯尔有限公司版权所有。
The in vivo recruitment of circulating host cells to the site to be regenerated is one of the promising strategies for therapeutic angiogenesis. Substance P (SP), a member of neuropeptides, mediates pain perception and regulates wound healing, inflammation, tumor cell proliferation, and angiogenesis. This SP enhanced the migration, adhesion, and angiogenic gene expression of granulocytes in vitro. A biodegradable hydrogel was prepared from an anionic derivative of gelatin to achieve the controlled release of SP in vivo. When the anionic gelatin hydrogels incorporating SP were subcutaneously implanted into the mouse back, significant angiogenesis was induced around the site implanted, in contrast to the injection of SP solution. In vivo accumulation of granulocytes around the implanted sites was observed. It is concluded that the controlled release of SP efficiently induced the recruitment and the subsequent activation of granulocytes, one of the circulating cells with angiogenic activities, from the blood circulation into the site implanted, resulting in enhanced angiogenesis. (C) 2010 Elsevier Ltd. All rights reserved.