Molecular phylogenetic studies on Theileria spp. isolates (China) based on small subunit ribosomal RNA gene sequences
Molecular phylogenetic studies on Theileria spp. isolates (China) based on small subunit ribosomal RNA gene sequences
复制标题
泰勒虫属的分子系统发育研究。
DOI：
10.1007/s11250-009-9392-x
复制
发表时间：
2010-01-01
影响因子：
1.7
通讯作者：
Zhao, Junlong L.
中科院分区：
文献类型：
--
作者：
Liu, Qin;Zhou, Yanqin Q.;Zhao, Junlong L.
6个泰勒虫属牛、布法罗和黑山羊中分离到的H2 O2。为了研究这些寄生虫的分类地位，进行了18 S rRNA基因的系统发育分析。通过聚合酶链反应扩增来自每个分离物的18 S rRNA基因，并对约1.75 kb的产物进行克隆和测序。对这些基因序列的系统发育分析表明，来自布法罗和牛的5种寄生虫属于瑟氏泰勒虫/东方泰勒虫群。湖北麻城山羊的寄生虫与我国北方绵羊分离的鲁温氏泰勒虫关系密切。这是利用分子生物学方法对泰勒虫进行分类的首次报道。结果表明，湖北省泰勒虫属（Theileria spp.）湖北省反刍动物泰勒虫属（Theileria spp.）
Six Theileria spp. from cattle, buffalo and black goat were identified in the Hubei province of China. In order to study the taxonomic status of these parasites, phylogenetic analysis of 18S rRNA genes were carried out. The 18S rRNA genes from each isolate were amplified by the polymerase chain reaction and the approximate 1.75 kb products were cloned and sequenced. Phylogenetic analysis of these gene sequences revealed that the five parasites from buffalo and cattle belonged to the Theileria sergenti/buffeli/orientalis group. The parasite from the Chinese goat (Macheng-Hubei, DQ286802) was closely related to Theileria luwenshuni isolated from sheep in the north of China. This represent the first report on the use of molecular phylogeny to classify Theileria spp. obtained in the Hubei province, showing that Theileria spp. from ruminants found in Hubei province belongs to the benign group of Theileria spp.