Molecular cloning and expression of a hexamerin cDNA from the malaria mosquito, Anopheles gambiae.
Molecular cloning and expression of a hexamerin cDNA from the malaria mosquito, Anopheles gambiae.
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疟疾蚊子冈比亚按蚊六聚体 cDNA 的分子克隆和表达。
DOI：
10.1111/j.1432-1033.1997.t01-1-00719.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Benes,H
中科院分区：
文献类型：
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作者：
Zakharkin,SO;Gordadze,AV;Korochkina,SE;Mathiopoulos,KD;DellaTorre,A;Benes,H
During the last larval instar, dipteran insects synthesize two hexamerins rich in aromatic residues, typified by the larval serum proteins 1 and 2 (LSP‐1 and LSP‐2) ofDrosophila melanogaster. We report here the characterization of a complete cDNA sequence encoding a LSP‐1‐like protein from a lower dipteran insect, the malaria mosquitoAnopheles gambiae. The cDNA encodes the subunit of a homohexamer,A. gambiaehexamerin‐1.1 (AgHex‐1.1), which is a major pupal protein but only a minor constituent of late larval hemolymph. AgHex‐1.1 is moderately rich in methionine (3.9%) and particularly rich in aromatic residues (21% Phe + Tyr). Cytogenetic analysis reveals AgHex‐1.1 to be encoded by a single‐copy gene localized to division 22F within the proximal 2La inversion breakpoint of chromosome 2 ofA. gambiae. The AgHex‐1.1 transcript is first detected in fourth‐instar larvae (L4) and disappears abruptly in early pupae.In situhybridization shows accumulation of the transcript uniquely in the larval fat body. AgHex‐1.1 mRNA is re‐expressed in male and female adults at about 10% of the L4 level, with no effect of bloodfeeding in females. The potential roles of AgHex‐1.1 inAnophelesdevelopment and reproductive maturation are discussed.
