Construction of protein-modified TiO2 nanoparticles for use with ultrasound irradiation in a novel cell injuring method.
Construction of protein-modified TiO2 nanoparticles for use with ultrasound irradiation in a novel cell injuring method.
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DOI：
10.1016/j.bmcl.2010.06.124
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发表时间：
2010-09
影响因子：
2.7
通讯作者：
C. Ogino;N. Shibata;R. Sasai;K. Takaki;Y. Miyachi;S. Kuroda;K. Ninomiya;N. Shimizu
中科院分区：
文献类型：
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作者：
C. Ogino;N. Shibata;R. Sasai;K. Takaki;Y. Miyachi;S. Kuroda;K. Ninomiya;N. Shimizu
最近，我们小组发现了一种替代二氧化钛（TiO2）活化方法，使用超声照射（US/TiO2）代替紫外线照射。利用氨基偶联方法将乙型肝炎病毒识别肝细胞的pre-S1/S2蛋白固定在tio2纳米颗粒表面。表面等离子体共振分析和免疫染色分析证实了蛋白质修饰的tio2纳米颗粒识别肝细胞的能力。HepG2癌细胞摄取tio2纳米颗粒后，采用US/ tio2法对细胞进行损伤；超声辐照强度为0.4W/cm2时，细胞损伤明显。结合这些结果，该策略可以在不久的将来应用于使用超声照射代替光动力治疗的新型细胞损伤系统。
Recently, our group discovered an alternative titanium dioxide (TiO2) activation method that uses ultrasound irradiation (US/TiO2) instead of ultraviolet irradiation. The pre-S1/S2 protein from hepatitis B virus, which recognizes liver cells, was immobilized to the surface of TiO2nanoparticles using an amino-coupling method. The ability of the protein-modified TiO2nanoparticles to recognize liver cells was confirmed by surface plasmon resonance analysis and immuno-staining analyses. After uptake of TiO2nanoparticles by HepG2 cancer cells, the cells were injured using this US/TiO2method; significant cell injury was observed at an ultrasound irradiation intensity of 0.4W/cm2. Together with these results, this strategy could be applied to new cell injuring systems that use ultrasound irradiation in place of photodynamic therapy in the near future.