A general tendency for conservation of protein length across eukaryotic kingdoms.
A general tendency for conservation of protein length across eukaryotic kingdoms.
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DOI：
10.1093/molbev/msh263
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发表时间：
2004-09
影响因子：
10.7
通讯作者：
Daryi Wang;Mu-Fen Hsieh;Wen-Hsiung Li
中科院分区：
文献类型：
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作者：
Daryi Wang;Mu-Fen Hsieh;Wen-Hsiung Li
蛋白质的伸长可以以多种方式发生，例如结构域的重复或插入，以及转座元件片段在编码区的募集，这被认为是蛋白质进化的一般趋势。事实上，先前的一项研究表明，酵母蛋白平均比细菌中的同源蛋白长，在这项研究中，我们发现酵母、线虫、果蝇、人和拟南芥中的蛋白质平均比它们在大肠杆菌中的同源蛋白长。然而，令人惊讶的是，我们发现真核生物王国的蛋白质序列长度是保守的。我们从酵母、线虫、果蝇、人和拟南芥中收集了1,252个同源蛋白质，发现这些蛋白质的总长度在这五个物种中非常相似，并且没有总的长度增加或减少的趋势。此外，虽然类似的蛋白质倾向于经历更多的序列长度变化，但也没有普遍的长度增加的趋势。然而，果蝇和人类共有的蛋白质，而不是酵母共有的蛋白质，平均来说比酵母、果蝇和人类共有的蛋白质要长得多。这是一个迫切需要答案的谜题。
Protein elongation can occur in many ways, such as domain duplication or insertion and as recruitment of a transposable element fragment into the coding region, and it is believed to be a general tendency in protein evolution. Indeed, a previous study showed that yeast proteins are, on average, longer than their orthologs in bacteria, and in this study, we found that proteins in yeast, nematode, Drosophila, human, and Arabidopsis are, on average, longer than their orthologs in Escherichia coli. Surprisingly, however, we found conservation of protein sequence length across eukaryotic kingdoms. We collected 1,252 orthologous proteins from yeast, nematode, Drosophila, human, and Arabidopsis and found that the total length of these proteins is very similar among the five species and that there is no general tendency for a protein to increase or decrease in length. Furthermore, although paralogous proteins tend to undergo more sequence-length changes, there is also no general tendency for length increase. However, proteins that are commonly shared by Drosophila and human but not by yeast are, on average, substantially longer than proteins that are shared by yeast, Drosophila, and human. This is a puzzle that begs for an answer.