Transient freezing regulates expression of extensin-tgpe genes in winter oilseed rape
Transient freezing regulates expression of extensin-tgpe genes in winter oilseed rape
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DOI：
10.1034/j.1399-3054.1998.1030214.x
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发表时间：
1998-06-01
影响因子：
6.4
通讯作者：
Kacperska, A
中科院分区：
文献类型：
--
作者：
Kozbial, PZ;Jerzmanowski, A;Kacperska, A
为了明确植物冷驯化过程的分子机制，我们检测了甘蓝型油菜中伸展蛋白类基因的表达。变种油树属叶，进行各种低温处理。北方分析表明，1260 nt和1450 nt的两个转录本与extA探针强烈杂交，并在经受瞬时冷冻处理的叶片中积累到高水平，引起约20%的膜损伤（对于未适应的叶片在-3 ℃下2小时，或对于冷适应的叶片在-12 ℃下2小时）。霜冻诱导的伸展素mRNA（尤其是1450-nt转录物）的积累明显高于机械创伤时观察到的积累。与非适应叶片相比，冷适应叶片中伸展素mRNA的霜冻依赖诱导在程度和持续时间上都显著升高。后孵育的冷驯化和霜冻处理的叶子在冰上，而不是在室温下延长了石灰过程中的伸展蛋白mRNA的积累，并导致显着增加的成绩单中的材料研究。可能参与的因素在诱导延伸蛋白基因转录进行了讨论，以及延伸蛋白细胞壁蛋白在植物对冻结的反应中的作用。
In order to identify the molecular mechanisms underlying the process of cold acclimation in plants, we examined the expression of extensin-type genes in Brassica napus L. var. oleifera L. leaves, subjected to various low temperature treatments. Northern analysis showed that two transcripts of 1260 nt and 1450 nt, strongly hybridized to the extA probe and accumulated to high levels in leaves subjected to transient freezing treatments, giving rise to ca 20% membrane injury (2 h at - 3 degrees C for non-acclimated or at - 12 degrees C for cold-acclimated leaves). The accumulation of the frost-induced extensin mRNAs (especially of the 1450-nt transcript) was markedly higher than that seen upon mechanical wounding. Frost-dependent induction of extensin mRNAs was markedly elevated both in extent and duration in cold-acclimated as compared to non-acclimated leaves. Post-incubation of cold-acclimated and frost-treated leaves on ice instead of at room temperature prolonged the lime course of extensin mRNA accumulation and resulted in a marked increase of the level of the transcripts in the material studied. Factors possibly involved in the induction of extensin gene transcription are discussed, as well as the role of extensin cell wall proteins in plant responses to freezing.