Development of Aβ terminal end-specific antibodies and sensitive ELISA for Aβ variant
Development of Aβ terminal end-specific antibodies and sensitive ELISA for Aβ variant
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DOI：
10.1016/j.bbrc.2004.05.051
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发表时间：
2004-07-02
影响因子：
3.1
通讯作者：
Matsuoka, Y
中科院分区：
文献类型：
--
作者：
Horikoshi, Y;Sakaguchi, G;Matsuoka, Y
阿尔茨海默病（AD）是一种与记忆功能障碍相关的神经退行性疾病。老年斑是AD的病理学标志，淀粉样蛋白β（Abeta）肽是这些斑的主要成分。Abeta肽来源于β-和γ-分泌酶对Abeta蛋白前体（APP）的蛋白水解裂解，产生两种主要物质，Abeta 1 -40和Abeta 1 -42。我们已经开发了针对这些肽的N-和C-末端的抗体，以及用于准确和灵敏的定量评估的ELISA。由N-末端（A β 1）末端特异性抗体、克隆82 E1和C-末端末端特异性抗体以及分别针对A β 40和A β 42的克隆1A 10和IC 3组成的夹心ELISA，检测全长A β 1 -40和1-42，灵敏度低于个位数fmol/ml C-末端抗体和针对Abeta中间区域的抗体的组合检测非转基因小鼠脑中的小鼠Abeta。(C)2004年爱思唯尔公司All rights reserved.
Alzheimer's disease (AD) is a neurodegenerative affliction associated with memory dysfunction. Senile plaques are a pathological hallmark of AD, and amyloid beta (Abeta) peptides are a major component of these plaques. Abeta peptides are derived from proteolytic cleavage of the Abeta protein precursor (APP) by beta- and gamma-secretases to generate two principal species, Abeta1-40 and Abeta1-42. We have developed antibodies against the N- and C-termini of these peptides, and an ELISA for accurate and sensitive quantitative assessment. Sandwich ELISA composed of N-terminus (Abeta1) end-specific antibody, clone 82E1, and C-termini end-specific antibodies, and clones 1A10 and IC3 for Abeta40 and Abeta42, respectively, detects full-length Abeta1-40 and 1-42 with a sensitivity in the sub single digit fmol/ml (equivalent to single digit pg/ml) range with no cross-reactivity to APP. A combination of C-termini antibodies and an antibody against the middle region of Abeta detects mouse Abeta in non-transgenic mouse brains. (C) 2004 Elsevier Inc. All rights reserved.