Rational and combinatorial design of peptide capping Ligands for gold nanoparticles
Rational and combinatorial design of peptide capping Ligands for gold nanoparticles
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DOI：
10.1021/ja0487269
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发表时间：
2004-08-18
影响因子：
15
通讯作者：
Fernig, DG
中科院分区：
文献类型：
--
作者：
Lévy, R;Thanh, NTK;Fernig, DG
基于对蛋白质折叠的考虑，设计了一种五肽配体CALNN，它可以将柠檬酸盐稳定的金纳米颗粒转化为非常稳定的水溶性金纳米颗粒，其化学性质类似于蛋白质。这些肽封端的金纳米颗粒可以冷冻干燥并储存为粉末，随后可以再溶解以产生稳定的水性分散体。过滤、尺寸排阻色谱、离子交换色谱、电泳和离心可应用于这些颗粒。研究了58种不同的肽序列对电解质诱导的纳米颗粒聚集的影响。这些肽配体赋予的稳定性取决于它们的长度、疏水性和电荷，并且在某些情况下导致与CALNN相比进一步改善的稳定性，从而产生肽封端配体的详细设计标准。提出了一种简单的识别基团的引入策略，并证明了生物素和Strep标签II。
Based on protein folding considerations, a pentapeptide ligand, CALNN, which converts citrate-stabilized gold nanoparticles into extremely stable, water-soluble gold nanoparticles with some chemical properties analogous to those of proteins, has been designed. These peptide-capped gold nanoparticles can be freeze-dried and stored as powders that can be subsequently redissolved to yield stable aqueous dispersions. Filtration, size-exclusion chromatography, ion-exchange chromatography, electrophoresis, and centrifugation can be applied to these particles. The effect of 58 different peptide sequences on the electrolyte-induced aggregation of the nanoparticles was studied. The stabilities conferred by these peptide ligands depended on their length, hydrophobicity, and charge and in some cases resulted in further improved stability compared with CALNN, yielding detailed design criteria for peptide capping ligands. A simple strategy for the introduction of recognition groups is proposed and demonstrated with biotin and Strep-tag II.