Extracellular levels of brain aspartate, glutamate and GABA during an inhibitory avoidance response in the rat
Extracellular levels of brain aspartate, glutamate and GABA during an inhibitory avoidance response in the rat
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DOI：
10.1111/j.1471-4159.2008.05452.x
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发表时间：
2008-08-01
影响因子：
4.7
通讯作者：
Giovannini, Maria Grazia
中科院分区：
文献类型：
--
作者：
Ballini, Chiara;Della Corte, Laura;Giovannini, Maria Grazia
采用微透析结合高效液相色谱法测定了大鼠前额叶皮层（mPFC）和腹侧海马（VH）细胞外天冬氨酸、谷氨酸和GABA的水平。谷氨酸的基础水平比天冬氨酸高约50%，GABA水平比兴奋性氨基酸低约20倍。两个脑区之间的任何三种氨基酸的基础水平没有显着差异。在VH和mPFC的采集和回忆试验期间，细胞外的天冬氨酸水平增加，而仅在VH采集期间，谷氨酸水平增加。GABA水平的显着增加，也检测到收购过程中，但仅在mPFC。通过反向透析将河豚毒素直接施用到mPFC或VH中，证明了天冬氨酸、谷氨酸和GABA的细胞外水平增加的神经元来源。这些发现，加上以前的证据，从我们和其他实验室，表明差异释放天冬氨酸和谷氨酸从兴奋性神经元的行为反应的性能，因此，不同的角色，两个兴奋性氨基酸应设想。
The extracellular levels of aspartate, glutamate and GABA were measured by microdialysis, coupled with an HPLC method, in rat prefrontal cortex (mPFC) and ventral hippocampus (VH) before and during the performance of a step-down inhibitory task. The basal levels of glutamate were about 50% higher than those of aspartate, and GABA levels were about 20-folds smaller than those of the excitatory amino acids. There were no significant differences in the basal levels of any of the three amino acids between the two brain regions. The extracellular levels of aspartate increased during acquisition and recall trials in both VH and mPFC, whereas those of glutamate increased in the VH during acquisition only. A significant increase in GABA levels was also detected during acquisition but only in the mPFC. The neuronal origin of the increased extracellular levels of aspartate, glutamate and GABA was demonstrated by administering tetrodotoxin directly into the mPFC or VH by reverse dialysis. These findings, together with previous evidence from our and other laboratories, indicate a differential release of aspartate and glutamate from excitatory neurons during the performance of behavioral responses, and therefore, distinct roles for the two excitatory amino acids should be envisaged.