Inflammatory cell infiltration as an indicator of Staphylococcus aureus infection and therapeutic efficacy in experimental mouse mastitis
Inflammatory cell infiltration as an indicator of Staphylococcus aureus infection and therapeutic efficacy in experimental mouse mastitis
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DOI：
10.1016/j.vetimm.2004.11.006
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发表时间：
2005-04-08
影响因子：
1.8
通讯作者：
Malouin, F
中科院分区：
文献类型：
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作者：
Brouillette, E;Grondin, G;Malouin, F
金黄色葡萄球菌在小鼠乳腺内定植诱导多形核中性粒细胞（PMNs）迁移，与牛乳腺炎期间观察到的相似。在本研究中，吖啶橙子染色，荧光显微镜和计算机辅助图像分析相结合的方法已被开发来定量PMN浸润在小鼠乳腺炎模型。这是使用石蜡包埋切片进行的，并且使用这种方法，我们表明PMNs的存在随着组织中存在的细菌数量而增加。与感染6 h的小鼠相比，感染12 h和24 h后乳腺组织中的PMN计数分别增加了近400倍和1100倍。抗生素头孢匹林10或25 mg/kg给药分别使PMN浸润减少71%和85%。总之，该方法可用于定量PMN浸润，作为感染组织切片中炎症和细菌负荷的标志物。(c)2004 Elsevier B. V.保留所有权利。
Staphylococcus aureus intramammary colonization of the mouse mammary gland induces migration of polymorphonuclear neutrophils (PMNs) similar to that observed during bovine mastitis. In the present study, a method combining acridine orange staining, fluorescence microscopy and computer-assisted image analysis has been developed to quantitate PMN infiltration in a mouse model of mastitis. This was carried out using paraffin embedded sections, and using this method, we showed that the presence of PMNs increased with the number of bacteria present in tissues. Nearly 400 and 1100 times more PMNs were counted in the mammary gland tissue after 12 and 24 h of infection, respectively, compared to mice infected for 6 h. Treatment with the antibiotic cephapirin at 10 or 25 mg/kg reduced PMN infiltration by 71 and 85%, respectively. In conclusion, this method can be used to quantitate PMN infiltration as a marker of inflammation and bacterial burden in infected tissue sections. (c) 2004 Elsevier B.V. All rights reserved.