Carriage of extended-spectrum β-lactamases in pig farmers is associated with occurrence in pigs
Carriage of extended-spectrum β-lactamases in pig farmers is associated with occurrence in pigs
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DOI：
10.1016/j.cmi.2015.05.032
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发表时间：
2015-10-01
影响因子：
14.2
通讯作者：
Heederik, D. J. J.
中科院分区：
文献类型：
--
作者：
Dohmen, W.;Bonten, M. J. M.;Heederik, D. J. J.
牲畜可能是广谱β -内酰胺酶产肠杆菌科（ESBL-PE）的储存库。本研究的目的是确定猪农、家庭成员和员工携带ESBL-PE的流行程度，以及其与猪携带的关系。从40个猪场的2388头猪（398个汇总样本）中采集直肠拭子，并从其中34个猪场的142名生活或工作人员中采集粪便样本。采用选择性镀（琼脂）法测定ESBL-PE的存在。采用PCR、微阵列分析、基因测序等方法对ESBL基因进行分析。采用多位点序列分型和基于pcr的复制子分型方法确定分离株的基因型和质粒。8人（6%）大肠杆菌中检测到ESBL基因(bla(CTX-M-1), n = 6；bla(-TEM-52), n = 1, bla(CTX-M-14), n = 1。在18个猪场的157株分离猪（107份合并样本）中（45%）检出ESBL基因(bla(CTX-M-1), n = 12；bla(-TEM-52), n = 6；bla(CTX-M-14), n = 3)。同一农场内的人和猪分离株具有相似的ESBL基因类型，并且在两个农场（例如大肠杆菌ST-453， bla(CTX-M-1), Inch）具有相同的序列和质粒类型，表明克隆传播。对于其余的农场，序列类型不同，但质粒类型不同。人类携带ESBL与每周在农场工作的平均小时数（OR = 1.04, 95% CI 1.02-1.06）和猪体内ESBL的存在（OR = 12.5, 95% CI 1.4-111.7）相关。每日接触携带ESBL- pe的猪与人类携带ESBL有关。临床微生物学与感染(C) 2015欧洲临床微生物学与传染病学会。Elsevier Ltd.出版。版权所有。
Livestock may serve as a reservoir for extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE). The objectives of this study were to determine the prevalence of carriage with ESBL-PE in pig farmers, family members and employees, and its association with carriage in pigs. Rectal swabs were taken from 2388 pigs (398 pooled samples) on 40 pig farms and faecal samples were obtained from 142 humans living or working on 34 of these farms. Presence of ESBL-PE was determined by selective plating (agar). ESBL genes were analysed by PCR or microarray analysis, and gene sequencing. Genotypes and plasmids were determined by multilocus sequence typing and PCR-based replicon typing for selected isolates. ESBL genes were detected in Escherichia coli from eight humans (6%) (bla(CTX-M-1), n = 6; bla(-TEM-52), n = 1 and bla(CTX-M-14), n = 1) on six farms. In 157 pig isolates (107 pooled samples) on 18 farms (45%) ESBL genes were detected (bla(CTX-M-1), n = 12; bla(-TEM-52), n = 6; and bla(CTX-M-14), n = 3). Human and pig isolates within the same farm harboured similar ESBL gene types and had identical sequence and plasmid types on two farms (e.g. E coli ST-453, bla(CTX-M-1), Inch), suggesting clonal transmission. For the remaining farms, sequence types, but not plasmid types, differed. Human ESBL carriage was associated with average number of hours working on the farm per week (OR = 1.04, 95% CI 1.02-1.06) and presence of ESBLs in pigs (OR = 12.5, 95% CI 1.4-111.7). Daily exposure to pigs carrying ESBL-PE is associated with ESBL carriage in humans. Clinical Microbiology and Infection (C) 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.