Subcutaneous Administration of PDGF-AA Improves the Functional Recovery After Spinal Cord Injury
Subcutaneous Administration of PDGF-AA Improves the Functional Recovery After Spinal Cord Injury
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皮下注射 PDGF-AA 可改善脊髓损伤后的功能恢复。
DOI：
10.3389/fnins.2019.00006
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发表时间：
2019-01-22
影响因子：
4.3
通讯作者：
Hu, Jian-Guo
中科院分区：
文献类型：
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作者：
Guo, Xue-Yan;Duan, Fei-Xiang;Hu, Jian-Guo
本课题组的前期研究表明，移植外源性血小板源性生长因子（PDGF）-AA过表达的少突胶质祖细胞（OPCs）可促进脊髓损伤（SCI）大鼠模型的组织修复和神经功能恢复。然而，目前尚不清楚PDGF-AA治疗是否也影响内源性少突胶质细胞（OL）甚至神经元，从而促进SCI后进一步的功能恢复。在本研究中，我们评估了脊髓损伤后立即直接皮下注射PDGF-AA的治疗潜力。我们证明，PDGF-AA注射导致增加的组织保留，髓鞘形成和功能恢复大鼠脊髓损伤后。进一步的实验证实PDGF-AA增加了内源性OPCs和OLs的存活，并促进OPCs的增殖和向OLs的分化。此外，PDGF-AA还保护受损脊髓中的运动神经元免于死亡。这些结果表明，PDGF-M管理可能是一种有效的治疗SCI。
Previous studies by our group have demonstrated that the transplantation of exogenous platelet-derived growth factor (PDGF)-AA-overexpressing oligodendrocyte progenitor cells (OPCs) promotes tissue repair and recovery of neurological function in a rat model of spinal cord injury (SCI). However, it remains unclear whether treatment with PDGF-AA also affects endogenous oligodendrocytes (OLs) or even neurons, thus promoting further functional recovery after SCI. In the present study, we evaluated the therapeutic potential of PDGF-AA treatment by direct subcutaneous injection of PDGF-AA immediately after SCI. We demonstrated that PDGF-AA injection resulted in increased tissue sparing, myelination and functional recovery in rats following SCI. Further experimentation confirmed that PDGF-AA increased the survival of endogenous OPCs and OLs, and promoted the proliferation of OPCs and their differentiation into OLs. Moreover, PDGF-AA also protected motor neurons from death in the injured spinal cord. These results indicated that PDGF-M administration may be an effective treatment for SCI.