Interferon-γ stimulates the expression of CX3CL1/fractalkine in cultured human endothelial cells
Interferon-γ stimulates the expression of CX3CL1/fractalkine in cultured human endothelial cells
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DOI：
10.1620/tjem.192.127
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发表时间：
2000-10-01
影响因子：
2.2
通讯作者：
Satoh, K
中科院分区：
文献类型：
--
作者：
Imaizumi, T;Matsumiya, T;Satoh, K
CX 3CL 1/Fractalkine是一种CX 3C趋化因子，是单核细胞和淋巴细胞趋化和粘附的有效激动剂。它首先被鉴定为用IL-1或TNF-alpha激活的内皮细胞中的膜蛋白。我们发现干扰素-γ（IFN-gamma）刺激的人脐静脉内皮细胞中fractalkine的表达增强。放线菌酮预处理细胞不抑制Fractalkine mRNA的表达。在细胞裂解物中发现了大部分fractalkine蛋白，并且抗体阻断实验公开了fractalkine有助于单核细胞粘附到用IFN-γ刺激的内皮单层。血管内皮细胞响应IFN-γ产生fractalkine，这可能通过引发单核细胞通过血管壁的运输在免疫应答中发挥重要作用。(C)2000东北大学医学出版社。
CX3CL1/Fractalkine, a CX3C chemokine, is a potent agonist for the chemotaxis and adhesion of monocytes and lymphocytes. It was first identified as a membrane protein in endothelial cells activated with IL-1 or TNF-alpha. We have found the enhanced expression of fractalkine in human umbilical vein endothelial cells stimulated with interferon-gamma (IFN-gamma). Pretreatment of the cells with cycloheximide did not inhibit the expression of fractalkine mRNA. The majority of fractalkine protein was found in the cell lysate, and an antibody-blocking experiment disclosed that fractalkine contributes to the adhesion of mononuclear cells to endothelial monolayers stimulated with IFN-gamma. Vascular endothelial cells produce fractalkine in response to IFN-gamma, and this may play an important role in immune responses by eliciting a traffic of mononuclear cells through the vascular wall. (C) 2000 Tohoku University Medical Press.