Stability of coagulation proteins in frozen plasma
Stability of coagulation proteins in frozen plasma
复制标题
DOI：
10.1097/00001721-200106000-00002
复制
发表时间：
2001-06-01
影响因子：
1.1
通讯作者：
Gourmelin, Y
中科院分区：
文献类型：
--
作者：
Woodhams, B;Girardot, O;Gourmelin, Y
本研究报告了正常柠檬酸血浆中所有常用凝血蛋白的冷冻稳定性：活化部分凝血活酶时间、凝血酶原时间（%）、凝血酶时间和纤维蛋白原（Clauss）；因子II、V、VII、VIII、IX、X、XI和XII的凝血试验；蛋白C（凝血）、蛋白S（凝血）、抗凝血酶（显色）和纤溶酶原（显色）的功能测定；血管性血友病因子和d -二聚体的免疫学检测。如果在零下24度或更低的温度下冷冻，所有这些因素都可以稳定保存3个月。在零下74度时，所有这些因素（允许10%的变化）至少稳定18个月，大多数稳定24个月。储存6个月后，蛋白质的数量在基线上显示出0.5%的变化，这表明即使在-74度的温度下也会发生一些腐烂。在-74度快速冷冻，然后在-24度储存，与在-24度冷冻和储存相比，没有明显的优势；因此，冻结过程本身并不对稳定性的丧失负责。当小样本（1ml）储存在死区最小的螺旋盖管中时，稳定性最好，特别是在-24度时。凝血蛋白稳定性的降低与温度和时间的影响直接相关。(C) 2001 Lippincott Williams & Wilkins。
This study reports on the frozen stability of all commonly measured coagulation proteins in normal citrated plasma: activated partial thromboplastin time, prothrombin time (%), thrombin time and fibrinogen (Clauss); clotting assays for factors II, V, VII, VIII, IX, X, XI and XII; functional assays for protein C (clotting), protein S (clotting), antithrombin (chromogenic) and plasminogen (chromogenic); and immunological assays for von Willebrand factor and D-dimer. All these factors listed are stable for up to 3 months if frozen at -24 degreesC or lower. At -74 degreesC, all these factors (allowing for 10% variation) were stable for at least 18 months, most were stable for 24 months. The number of proteins showing > 5% variation over baseline after 6 months storage indicates that some decay does occur even at -74 degreesC. There was no clear advantage in snap freezing at -74 degreesC and then storing at -24 degreesC over both freezing and storing at -24 degreesC; therefore, the freezing process itself is not responsible for the loss of stability. The best stability, especially at -24 degreesC, was obtained when small samples (1 ml) were stored in screw-cap tubes with a minimum dead space. The decrease in stability of the coagulation proteins directly correlates with the effect of temperature and time. (C) 2001 Lippincott Williams & Wilkins.