Selection of rodent species appropriate for mtDNA transfer to generate transmitochondrial mito-mice expressing mitochondrial respiration defects.
Selection of rodent species appropriate for mtDNA transfer to generate transmitochondrial mito-mice expressing mitochondrial respiration defects.
复制标题
DOI：
10.1538/expanim.63.21
复制
发表时间：
2014
影响因子：
2.4
通讯作者：
Hayashi J
中科院分区：
文献类型：
--
作者：
Enoki S;Shimizu A;Hayashi C;Imanishi H;Hashizume O;Mekada K;Suzuki H;Hashimoto T;Nakada K;Hayashi J
先前的报道表明，携带小家鼠核DNA和褐家鼠mtDNA的线粒体有丝分裂小鼠不表达呼吸缺陷，而携带褐家鼠mtDNA的ES杂种不能产生携带褐家鼠mtDNA的线粒体有丝分裂小鼠，因为会诱导显著的呼吸缺陷并导致多能性丧失。本研究从褐家鼠和褐家鼠两种啮齿类动物中分离出线粒体线粒体杂交体，并对其氧耗率进行了比较。结果表明，系统发育距离与氧耗率降低之间存在较强的负相关关系，这可能是由于鼠核基因组和线粒体基因组的共同进化，以及由此导致的鼠核基因组与其他啮齿类动物线粒体基因组的不相容。这些观察结果表明，m.c caroli是产生线粒体有丝分裂小鼠的合适的线粒体DNA供体。然后，我们用M. caroli mtDNA产生ES cybrids，发现这些ES cybrids表达呼吸缺陷而不失去多能性，可以用于产生表达线粒体疾病的线粒体线粒体分裂小鼠。
Previous reports have shown that transmitochondrial mito-mice with nuclear DNA from Mus musculus and mtDNA from M. spretus do not express respiration defects, whereas those with mtDNA from Rattus norvegicus cannot be generated from ES cybrids with mtDNA from R. norvegicus due to inducing significant respiration defects and resultant losing multipotency. Here, we isolated transmitochondrial cybrids with mtDNA from various rodent species classified between M. spretus and R. norvegicus, and compared the O2 consumption rates. The results showed a strong negative correlation between phylogenetic distance and reduction of O2 consumption rates, which would be due to the coevolution of nuclear and mitochondrial genomes and the resultant incompatibility between the nuclear genome from M. musculus and the mitochondrial genome from the other rodent species. These observations suggested that M. caroli was an appropriate mtDNA donor to generate transmitochondrial mito-mice with nuclear DNA from M. musculus. Then, we generated ES cybrids with M. caroli mtDNA, and found that these ES cybrids expressed respiration defects without losing multipotency and can be used to generate transmitochondrial mito-mice expressing mitochondrial disorders.
登录
查看更多内容
影响因子：
56.9
作者：
Fan, Weiwei;Waymire, Katrina G.;Wallace, Douglas C.
通讯作者：
Wallace, Douglas C.
DOI：
10.1038/nrg1606
发表时间：
2005-05
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Isobe, K;Ito, S;Hayashi, JI
通讯作者：
Hayashi, JI
影响因子：
10.7
作者：
McKenzie, M;Chiotis, M;Trounce, IA
通讯作者：
Trounce, IA
影响因子：
4.8
作者：
Inoue, K;Ito, S;Hayashi, JI
通讯作者：
Hayashi, JI