Methanocaldococcus jannaschii uses a modified mevalonate pathway for biosynthesis of isopentenyl diphosphate
Methanocaldococcus jannaschii uses a modified mevalonate pathway for biosynthesis of isopentenyl diphosphate
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DOI：
10.1128/jb.188.9.3192-3198.2006
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发表时间：
2006-05-01
影响因子：
3.2
通讯作者：
White, RH
中科院分区：
文献类型：
--
作者：
Grochowski, LL;Xu, HM;White, RH
古细菌已被证明可以从甲羟戊酸产生类异戊二烯;然而，基因组分析未能根据序列相似性识别甲羟戊酸途径中的几个基因。由MJ 0044基因编码的预测古细菌激酶与古细菌中的其他甲羟戊酸途径基因相关，并且预测为“缺失的”磷酸甲羟戊酸激酶。测试了MJ 0044衍生的蛋白的磷酸新戊酸激酶活性，发现其不催化该反应。发现MJ 0044基因产物磷酸化异戊烯基磷酸，产生异戊烯基二磷酸。与其他已知的与异戊二烯生物合成相关的激酶不同，詹氏甲烷醛球菌异戊烯基磷酸激酶被预测为异戊糖激酶超家族的成员。
Archaea have been shown to produce isoprenoids from mevalonate; however, genome analysis has failed to identify several genes in the mevalonate pathway on the basis of sequence similarity. A predicted archaeal kinase, coded for by the MJ0044 gene, was associated with other mevalonate pathway genes in the archaea and was predicted to be the "missing" phosphornevalonate kinase. The MJ0044-derived protein was tested for phosphornevalonate kinase activity and was found not to catalyze this reaction. The MJ0044 gene product was found to phosphorylate isopentenyl phosphate, generating isopentenyl diphosphate. Unlike other known kinases associated with isoprene biosynthesis, Methanocaldococcus jannaschii isopentenyl phosphate kinase is predicted to be a member of the aspartokinase superfamily.