Cell spreading and lamellipodial extension rate is regulated by membrane tension.
Cell spreading and lamellipodial extension rate is regulated by membrane tension.
复制标题
细胞扩散和层状扩展率受膜张力调节。
DOI：
10.1083/jcb.148.1.127
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发表时间：
2000-01-10
影响因子：
7.8
通讯作者：
Sheetz, M P
中科院分区：
文献类型：
--
作者：
Raucher, D;Sheetz, M P
细胞的伸展和运动需要质膜的伸展和肌动蛋白的组装。在体外，伸展必须克服质膜内张力的阻力。我们在此报道，加入两亲性化合物或荧光脂类来扩张质膜，增加了细胞铺展和片脂延伸的速度，刺激了新的片脂延伸，并导致了表观膜张力的降低。此外，在PDGF刺激的运动中，片脂延伸率的增加与表观膜张力的降低以及通过磷脂酰肌醇二磷酸的水解降低膜-细胞骨架的粘附性有关。相反，当渗透膨胀细胞增加膜张力时，延伸率降低。因此，我们认为膜脂伸展过程可以被物理信号激活(可能是次要的)，伸展的速度直接依赖于质膜的张力。定量分析表明，膜脂伸长率与表观膜张力呈负相关。这些研究描述了一种物理化学机制，涉及通过磷脂酰肌醇-4，5-二磷酸-蛋白质相互作用改变膜-细胞骨架的粘附性，以调节和刺激推动片脂伸展的生化过程。
Cell spreading and motility require the extension of the plasma membrane in association with the assembly of actin. In vitro, extension must overcome resistance from tension within the plasma membrane. We report here that the addition of either amphiphilic compounds or fluorescent lipids that expanded the plasma membrane increased the rate of cell spreading and lamellipodial extension, stimulated new lamellipodial extensions, and caused a decrease in the apparent membrane tension. Further, in PDGF-stimulated motility, the increase in the lamellipodial extension rate was associated with a decrease in the apparent membrane tension and decreased membrane–cytoskeleton adhesion through phosphatidylinositol diphosphate hydrolysis. Conversely, when membrane tension was increased by osmotically swelling cells, the extension rate decreased. Therefore, we suggest that the lamellipodial extension process can be activated by a physical signal (perhaps secondarily), and the rate of extension is directly dependent upon the tension in the plasma membrane. Quantitative analysis shows that the lamellipodial extension rate is inversely correlated with the apparent membrane tension. These studies describe a physical chemical mechanism involving changes in membrane–cytoskeleton adhesion through phosphatidylinositol 4,5-biphosphate–protein interactions for modulating and stimulating the biochemical processes that power lamellipodial extension.