ELIMINATION OF VAGINAL COLONIZATION WITH ESCHERICHIA-COLI BY ADMINISTRATION OF INDIGENOUS FLORA
ELIMINATION OF VAGINAL COLONIZATION WITH ESCHERICHIA-COLI BY ADMINISTRATION OF INDIGENOUS FLORA
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DOI：
10.1128/iai.57.8.2447-2451.1989
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发表时间：
1989-08-01
影响因子：
3.1
通讯作者：
WINBERG, J
中科院分区：
文献类型：
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作者：
HERTHELIUS, M;GORBACH, SL;WINBERG, J
在4只成年食蟹猴中建立了阿莫西林给药诱导的致肾盂肾炎大肠埃希菌菌株的持续阴道定殖。这种定植模仿了在易患尿路感染的女性中观察到的定植。试图消除E。大肠杆菌定殖并恢复正常条件。给予乳酸杆菌混悬液或来自健康未操作猴的阴道液，重复阴道冲洗5至9天。完全消除阴道E。在六个使用乳酸杆菌的实验中的两个中观察到大肠杆菌的感染，在其他四个实验中观察到减少。冲洗阴道液可消除大肠杆菌，效果较好。大肠杆菌定殖率为80%。在其中两个，一个单一的冲洗足以获得非殖民化。新鲜阴道液清除E.阴道中的大肠杆菌可以从一只猴子转移到另一只猴子身上。本研究证实了正常植物群在抵抗潜在致病性粘附性大肠杆菌生殖器定植中的作用。杆菌这些发现可能的临床相关性必须进一步检查。
A persistent vaginal colonization with a pyelonephritogenic strain of Escherichia coli, induced by administration of amoxicillin, was established in four adult cynomolgus monkeys. This colonization mimicked the one seen in urinary tract infection-prone human females. Attempts to eliminate the E. coli colonization and restore normal conditions were made. Either suspensions of lactobacilli or vaginal fluid from a healthy unmanipulated monkey was administered as repeated vaginal flushes for 5 to 9 days. A total elimination of vaginal E. coli was observed in two of six experiments with lactobacilli, and a decrease was observed in the other four. A better result was obtained with flushes of vaginal fluid, which eliminated the E. coli colonization in eight of eight experiments. In two of these, a single flush was sufficient to obtain a decolonization. The ability of fresh vaginal fluid to eliminate E. coli from the vagina could be transferred from one monkey to another. This study demonstrates the role of the normal flora in the defense against genital colonization with potentially pathogenic adhering E. coli. The possible clinical relevance of these findings must be further examined.