Influence of Gender and SNPs in GPX1 Gene on Biomarkers of Selenium Status in Healthy Brazilians.
Influence of Gender and SNPs in GPX1 Gene on Biomarkers of Selenium Status in Healthy Brazilians.
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DOI：
10.3390/nu8050081
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发表时间：
2016-05-05
期刊：
影响因子：
5.9
通讯作者：
Cozzolino SM
中科院分区：
文献类型：
--
作者：
Donadio JL;Guerra-Shinohara EM;Rogero MM;Cozzolino SM
由于土壤硒含量的自然变化、饮食模式和snp的存在，硒（Se）状况在世界范围内存在差异。此外，巴西人的硒状况及其遗传变异与硒生物标志物的关系尚不清楚。这项工作调查了健康巴西人谷胱甘肽过氧化物酶基因snp与硒状态生物标志物之间的关系。这项研究是在巴西圣保罗的116名健康成年人中进行的。采用血红蛋白原子吸收光谱法测定血浆和红细胞硒含量。用生化分析仪测定红细胞GPx （eGPx）活性。采用Taqman®法实时PCR检测基因型。eGPx活性在雌性中高于雄性。GPX1 rs8179169杂合GC携带者红细胞Se浓度较低。除rs8179169外，普通基因型的雌性eGPx活性较高。rs8179169基因GC携带者的红细胞硒含量在两性中均较低，只有rs1050450和rs1800668变异等位基因的男性携带者具有较高的eGPx活性。总之，在这项以健康巴西人为研究对象的试点研究中，GPX1 snp的基因型和性别影响硒状态的生物标志物。
Selenium (Se) status varies worldwide as a result of natural variation of Se content in soils, dietary pattern, and the presence of SNPs. Further, Se status in Brazilians and its relationship between genetic variation and Se biomarkers is unknown. This work investigated the association between SNPs in glutathione peroxidase genes and biomarkers of Se status in healthy Brazilians. The study was conducted in 116 healthy adults in São Paulo, Brazil. Plasma and erythrocyte Se were measured by HGFAAS. Erythrocyte GPx (eGPx) activity was measured spectrometrically in a biochemical analyzer. Genotypes were determined by real-time PCR using Taqman® Assays. eGPx activity was higher in females compared with males. Lower erythrocyte Se concentrations were found in heterozygous GC carriers for GPX1 rs8179169. eGPx activity was higher in females with the common genotypes, except for rs8179169. GC carriers for rs8179169 had lower erythrocyte Se in both genders, and only male carriers of the variant alleles of both rs1050450 and rs1800668 had higher eGPx activity. In conclusion, the genotype for SNPs in GPX1 and gender affected biomarkers of Se status in this pilot study with healthy Brazilians.