Comprehensive characterization of oil refinery effluent-derived humic substances using various spectroscopic approaches.
Comprehensive characterization of oil refinery effluent-derived humic substances using various spectroscopic approaches.
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DOI：
10.1016/j.chemosphere.2005.01.012
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发表时间：
2005-07
期刊：
影响因子：
8.8
通讯作者：
Li Lingbo;Yan Song;Han Congbi;Shan Guangbo
中科院分区：
文献类型：
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作者：
Li Lingbo;Yan Song;Han Congbi;Shan Guangbo
炼油废水腐殖质(HS)对于发展炼油废水回用技术和研究废水的环境化学具有重要意义。采用便携式反渗透(RO)系统对炼油厂废水中的溶解有机物(DOM)进行了浓缩处理。用XAD-8树脂从RO保留物中分离得到HS。采用254 nm比紫外吸收光谱(SUV254)、元素分析、尺寸排除色谱(SEC)、固态交叉极化魔角旋转13C核磁共振(13C CPMAS)、傅立叶变换红外光谱(FTIR)和电喷雾电离/离子陷阱/质谱仪(ESI/Ion Trap/MS)等方法对HS进行了表征。便携式反渗透系统对浓缩炼油厂废水具有较高的产率和DOM回收率。炼油厂出水中溶解有机碳(DOC)浓度为9.9 mg/L，其中胡敏酸(HA)和黄腐酸(FA)分别占2.3%和34.6%。元素分析和SUV254分析表明，炼油厂排出的HS中脂肪结构含量较高，芳香族结构含量较低。炼油厂出水衍生的HS比天然HS的相对分子质量低。经SEC测定，HA的数均相对分子质量和重均相对分子质量分别为1069和2934，FA的数均相对分子质量和重均相对分子质量分别为679和1212。经ESI/离子陷阱/MS测定，Mn和Mwof FA分别为330和383。通过~(13)C核磁共振分析，发现炼油厂出水HS中含有四种碳结构(脂肪族、芳香族、杂脂肪族和羧基碳)。定量结果支持这些HS富含脂肪族碳，贫芳香族碳的解释。通过FTIR分析，确定了炼油厂出水HS中的蛋白质类物质。
Refinery effluent-derived humic substances (HS) are important for developing refinery effluent reclamation techniques and studying the environmental chemistry of wastewater effluents. In this study, dissolved organic matter (DOM) from refinery effluent was concentrated using a portable reverse osmosis (RO) system. HS were isolated from RO retentates with XAD-8 resin. A variety of approaches such as specific UV absorbance at 254nm (SUV254), elemental analysis, size exclusion chromatography (SEC), solid-state cross polarization magic angle spinning13C nuclear magnetic resonance spectrometry (13C CPMAS NMR), Fourier transform infrared spectrometry (FTIR), and electrospray ionization/ion trap/mass spectrometry (ESI/ion trap/MS) were employed for characterization of HS. The portable RO system exhibited high yield and recovery of DOM for concentrating refinery effluent. The concentration of dissolved organic carbon (DOC) in the refinery effluent was 9.9mg/l, in which humic acids (HA) and fulvic acids (FA) accounted for 2.3% and 34.6%, respectively. Elemental and SUV254analyses indicated relative high amounts of aliphatic structures and low amounts of aromatic structures in refinery effluent-derived HS. Refinery effluent-derived HS displayed lower molecular weight than natural HS. The number-average molecular weight (Mn) and the weight-average molecular weight (Mw) of HA were 1069 and 2934, and those of FA were 679 and 1212 by SEC, respectively. By ESI/ion trap/MS, the Mnand the Mwof FA were 330 and 383. Four kinds of carbon structures (aliphatic, aromatic, heteroaliphatic, and carboxylic carbons) were found in refinery effluent-derived HS by13C NMR analysis. The quantitative results support the interpretation that these HS are rich in aliphatic carbons and poor in aromatic carbons. Proteinaceous materials were identified by FTIR analysis in refinery effluent-derived HS.