A novel role for proliferin-2 in the ex vivo expansion of hematopoietic stem cells
A novel role for proliferin-2 in the ex vivo expansion of hematopoietic stem cells
复制标题
DOI：
10.1016/s0014-5793(03)00844-5
复制
发表时间：
2003-08-28
期刊：
影响因子：
3.5
通讯作者：
Lodish, HF
中科院分区：
文献类型：
--
作者：
Choong, ML;Tan, MCL;Lodish, HF
在造血支持基质细胞系的微阵列分析中发现了一个增殖素基因家族。增殖素-2 （PLF2）增加了MS5.1饲养层培养的原代造血干细胞（HSC）富集骨髓细胞的长期培养起始细胞（LTC-IC）的频率，从1 / 340增加到1 / 256。重复使用AFT024喂料层也显示出类似的LTG-IC增加（从386个细胞中1个增加到260个细胞中1个）。LTC-ICs的克隆输出量也显著增加。通过细胞计数和DNA合成测定，发现经PLF2处理的各种造血和基质细胞系的生长增加了4-27%。这些发现开辟了使用PLF2作为生长因子鸡尾酒的新成员来体外扩增HSC的可能性。(C) 2003年由Elsevier B.V.代表欧洲生化学会联合会出版。
A family of proliferin genes was discovered on a microarray analysis of hematopoiesis supportive stromal cell lines. Proliferin-2 (PLF2) increased the frequency of long-term culture-initiating cells (LTC-IC) from 1 in 340 to 1 in 256 of the primary hematopoietic stem cell (HSC)-enriched bone marrow cells grown on MS5.1 feeder layer. A repeat using AFT024 feeder layer also showed a similar increase in LTG-IC (from 1 in 386 cells to 1 in 260 cells). The clonogenic output of the LTC-ICs was also increased significantly. The growth of various hematopoietic and stromal cell lines treated with PLF2 was found to increase by 4-27%, as measured by cell count and DNA synthesis assay. These findings open up the possibility of using PLF2 as a new member of the growth factor cocktails for the ex vivo expansion of HSC. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.