3D Cell Printing of Perfusable Vascularized Human Skin Equivalent Composed of Epidermis, Dermis, and Hypodermis for Better Structural Recapitulation of Native Skin
3D Cell Printing of Perfusable Vascularized Human Skin Equivalent Composed of Epidermis, Dermis, and Hypodermis for Better Structural Recapitulation of Native Skin
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DOI：
10.1002/adhm.201801019
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发表时间：
2019-04-11
影响因子：
10
通讯作者：
Cho, Dong-Woo
中科院分区：
文献类型：
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作者：
Kim, Byoung Soo;Gao, Ge;Cho, Dong-Woo
尽管皮肤细胞打印已经显示出制造功能性皮肤等同物的前景，但通过3D细胞打印的现有皮肤模型仍然由真皮和表皮层组成。然而，打印皮肤的一个关键希望是改善人类皮肤的结构复杂性，使其能够精确定位多种细胞类型和生物材料。在这里，使用3D细胞打印增加了皮肤解剖的复杂性。提出了一种新的打印平台，用于工程化由表皮、真皮和皮下组织组成的成熟的可灌注血管化3D人类皮肤等效物。使用代表表皮、真皮和皮下组织的每个区域的功能标记物来评估皮肤模型以确认组织成熟。据推测，血管化的真皮和皮下隔室提供了一个更真实的微环境，可以促进与表皮隔室的交叉对话，产生更好的表皮形态发生的重演。研究上皮组织中的皮肤干性。这些发现揭示了与真皮和表皮皮肤模型相比，全层皮肤与天然人类皮肤具有更多的相似性，表明它更好地反映了天然人类皮肤的实际复杂性。可以预见，它提供了更好的预测性和可靠的体外平台的病理研究和皮肤疾病建模的机制调查。
Although skin cell-printing has exhibited promises for fabrication of functional skin equivalents, existing skin models through 3D cell printing are still composed of dermal and epidermal layers. However, a key hope for printing skin is to improve structural complexity of human skin over conventional construction, enabling the precise localization of multiple cell types and biomaterials. Here, the complexity of skin anatomy is increased using 3D cell printing. A novel printing platform is suggested for engineering a matured perfusable vascularized 3D human skin equivalent composed of epidermis, dermis, and hypodermis. The skin model is evaluated using functional markers representing each region of epidermis, dermis, and hypodermis to confirm tissue maturation. It is hypothesized that the vascularized dermal and hypodermal compartments that provide a more realistic microenvironment can promote cross-talks with the epidermal compartment, producing better recapitulation of epidermal morphogenesis. Skin stemness in epithelial tissue is investigated. These findings reveal that the full-thickness skin has more similarities to the native human skin compared with the dermal and epidermal skin model, indicating that it better reflects the actual complexity of native human skin. It is envisioned that it offers better predictive and reliable in vitro platform for investigation of mechanisms of pathological research and skin disease modeling.