Genetic variants of apolipoprotein A5 T-1131C and apolipoprotein E common polymorphisms and their relationship to features of metabolic syndrome in adult dyslipidemic patients
Genetic variants of apolipoprotein A5 T-1131C and apolipoprotein E common polymorphisms and their relationship to features of metabolic syndrome in adult dyslipidemic patients
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DOI：
10.1016/j.clinbiochem.2014.03.015
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发表时间：
2014-08-01
影响因子：
2.8
通讯作者：
Malina, Pavel
中科院分区：
文献类型：
--
作者：
Novotny, Dalibor;Vaverkova, Helena;Malina, Pavel
目的：目的是评估T-1131 C载脂蛋白A5（Apo A5）基因（rs662799）多态性变体和载脂蛋白E（Apo E）基因常见多态性变体（rs 429358，rs7412）与代谢综合征（MetS）的体征的关系。我们检测了590例无症状血脂异常患者，根据NCEP ATP III Panel标准分为MetS+组（n = 146）和MetS-组（n = 444）。我们评估了基因型频率和各组之间MetS特征的差异。结果：MetS+组和MetS-组Apo A5和Apo E基因型和等位基因频率无统计学差异。在所有受试者和MetS组中，我们证实了-1131C Apo A5次要等位基因与甘油三酯升高的众所周知的关联（TG，p < 0.001）。与E33常见变异体相比，Apo E基因E2和E4变异体与更高水平的TG相关（p < 0.01）。然而，在MetS+受试者中没有观察到统计学差异，无论该组中的TG水平是否显著较高。所有血脂异常患者的Apo A5/Apo E变异分析显示，与常见的-1131 T/E3变异携带者相比，所有亚组中的TG水平均显著升高，其中-1131 C/E4变异亚组中的升高最多。Logistic回归分析模型显示，载脂蛋白A5，载脂蛋白E和所有载脂蛋白A5/载脂蛋白E变异体与代谢综合征，即使调整年龄和性别后，没有关联：结论：我们的研究细化载脂蛋白A5和载脂蛋白E遗传变异体在成人血脂异常患者组中的作用。结果表明，除TG外，Apo A5 T-1131 C（rs662799）和Apo E（rs 429358，rs7412）多态性对MetS特征无显著影响。(C)2014年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objectives: The aim was to evaluate the relationships of the T-1131C (rs662799) polymorphism variants of apolipoprotein A5 (Apo A5) gene and variants of apolipoprotein E (Apo E) gene common polymorphism (rs429358, rs7412) to signs of metabolic syndrome (MetS).Design and methods: We examined 590 asymptomatic dyslipidemic patients divided into MetS+ (n = 146) and MetS- (n = 444) groups according to criteria of NCEP ATPIII Panel. We evaluated genotype frequencies and differences in MetS features between individual groups. Logistic regression analysis was used for the evaluation of Apo A5/Apo E variants as possible risk factors for MetS.Results: We found no statistical differences between genotype and allele frequencies for both Apo A5 and Apo E polymorphisms between MetS+ and MetS- groups. In all subjects and MetS- group, we confirmed well-known association of the - 1131C Apo A5 minor allele with elevated triglycerides (TG, p < 0.001). The Apo E gene E2 and E4 variants were associated with higher levels of TG (p < 0.01) in comparison to E33 common variant. However, no statistical differences were observed in MetS+ subjects, regardless of significantly higher TG levels in this group. Apo A5/Apo E variant analysis in all dyslipidemic patients revealed significant increase of TG levels in all subgroups in comparison to common - 1131T/E3 variant carriers, the most in - 1131C/E4 variant subgroup. Logistic regression analysis models showed no association of Apo A5, Apo E and all Apo A5/Apo E variants with metabolic syndrome, even after adjustment for age and sex.Conclusion: Our study refined the role of Apo A5 and Apo E genetic variants in the group of adult dyslipidemic patients. We demonstrate that except of TG, Apo A5 T-1131C (rs662799) and Apo E (rs429358, rs7412) polymorphisms have no remarkable effect on MetS characteristics. (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.