Detection of in vivo synthesis of polycistronic mRNAs of vesicular stomatitis virus.
Detection of in vivo synthesis of polycistronic mRNAs of vesicular stomatitis virus.
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水泡性口炎病毒多顺反子mRNA体内合成的检测。
DOI：
10.1016/0042-6822(84)90297-6
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Samuel,CE
中科院分区：
文献类型：
--
作者：
Masters,PS;Samuel,CE
采用RNA印迹杂交技术检测了水疱性口炎病毒多顺反子转录本在人羊膜U细胞和小鼠L细胞中的内合成。在凝胶电泳系统确定的分子量范围内，通过杂交模式和表观分子量，观察到序列连接信息的所有可能组合。放线菌素D预处理小鼠L细胞不影响多反onic信息的频率和大小，在L细胞和U细胞之间也没有差异。水疱性口炎病毒多反链转录本在体内的合成基本均匀，但NS-M双反链转录本的合成频率要高得多。大多数多反式RNA都是多(A)+，但至少有一种四反式分子N-NS-M-G明显是多(A)−。在寡核苷酸（dT）存在的情况下，用RNase H处理后的RNA分析表明，它们体内合成的多(A)+多顺反子物种NS-M、M-G和N-NS-M在它们的3 '分子末端有多(A)束，但在它们的反反子连接处没有。
Thein vivosynthesis of polycistronic transcripts of vesicular stomatitis virus in human amnion U cells and mouse L cells was detected by RNA blot hybridization. Within the molecular weight range resolved by this gel electrophoresis system, all possible combinations of sequentially linked messages were observed, as identified by their patterns of hybridization and their apparent molecular weights. Actinomycin D pretreatment of mouse L cells did not affect the frequency or size of polycistronic messages, nor did these differ between L cells and U cells. Vesicular stomatitis virus polycistronic transcripts were synthesizedin vivoin a roughly uniform distribution, except for the NS-M dicistronic mRNA, which was much more frequent. Most of the polycistronic RNA species were found to be poly(A)+, but at least one, the tetracistronic molecule N-NS-M-G, was clearly poly(A)−. Analysis of RNA following treatment with RNase H in the presence of oligo(dT) indicated that thein vivo-synthesized poly(A)+polycistronic species NS-M, M-G, and N-NS-M had poly(A) tracts at their 3′ molecular termini but not internally at their intercistronic junctions.