Spherosomes of Castor Bean Endosperm: MEMBRANE COMPONENTS, FORMATION, AND DEGRADATION.
Spherosomes of Castor Bean Endosperm: MEMBRANE COMPONENTS, FORMATION, AND DEGRADATION.
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蓖麻子胚乳球体：膜成分、形成和降解。
DOI：
10.1104/pp.65.6.1176
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发表时间：
1980
期刊：
影响因子：
7.4
通讯作者：
A. Huang
中科院分区：
文献类型：
--
作者：
R. Moreau;K. Liu;A. Huang
对蓖麻球的膜成分进行了表征。用乙醚提取球形体中储存的三酰甘油，蔗糖梯度离心分离球形体的膜。它的表观平衡密度为1.12克/立方厘米，并具有抗霉素A不敏感的NADH细胞色素c还原酶和酸性脂肪酶。磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰肌醇大致等量是主要的磷脂。用丙烯酰胺凝胶电泳将膜蛋白分离成分子量在10，000 ~ 70，000之间的几条主要和次要蛋白带，其蛋白质谱型不同于内质网、线粒体和乙醛酸酶体膜。一个惊人的相似性存在于每个球体组件的发育模式。这一发现表明，球体是作为一个单独的单位合成和降解的。从成熟的种子中分离的球状体表现出快速水解的存储脂质在体外，从而提高了在种子成熟过程中防止在体内自溶的球状体的细胞控制的问题。
The membrane components of the castor bean spherosomes were characterized. The storage triacylglycerols of isolated spherosomes were extracted with diethyl ether, and the membrane was isolated by sucrose gradient centrifugation. It had an apparent equilibrium density of 1.12 grams per cubic centimeter, and possessed an antimycin A-insensitive NADH cytochrome c reductase and an acid lipase. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol in roughly equal amounts were the major phospholipids. The membrane proteins were resolved into several major and minor protein bands of molecular weights ranging from 10,000 to 70,000 by acrylamide gel electrophoresis, and the protein pattern in the gel was different from those of the endoplasmic reticulum, mitochondrial, and glyoxysomal membranes.The varying amounts of spherosomal components in the seed were followed throughout seed maturation and germination. A striking similarity existed in the developmental pattern of each of the spherosomal components. This finding suggests that the spherosome is synthesized and degraded as one individual unit. The spherosomes isolated from maturing seeds exhibited rapid hydrolysis of the storage lipid in vitro, thus raising the problem of cellular control in preventing in vivo autolysis of the spherosomes during seed maturation.