Tuning riboswitch regulation through conformational selection.
Tuning riboswitch regulation through conformational selection.
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DOI：
10.1016/j.jmb.2010.10.056
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发表时间：
2011-01-28
影响因子：
5.6
通讯作者：
Foster MP
中科院分区：
文献类型：
--
作者：
Wilson RC;Smith AM;Fuchs RT;Kleckner IR;Henkin TM;Foster MP
The SMK box riboswitch, which represents one of three known classes of S-adenosylmethionine (SAM)-responsive riboswitches, regulates gene expression in bacteria at the level of translation initiation. In contrast to most riboswitches, which contain separate domains responsible for ligand recognition and gene regulation, the ligand-binding and regulatory domains of the SMK box riboswitch are coincident. This property was exploited to allow the first atomic-level characterization of a functionally intact riboswitch in both the ligand-bound and ligand-free states. NMR spectroscopy revealed distinct mutually exclusive RNA conformations that are differentially populated in the presence or absence of the effector metabolite. Isothermal titration calorimetry and in vivo reporter assay results revealed the thermodynamic and functional consequences of this conformational equilibrium. We present a comprehensive model of the structural, thermodynamic, and functional properties of this compact RNA regulatory element.
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