Over- and under-expressed microRNAs in human colorectal cancer
Over- and under-expressed microRNAs in human colorectal cancer
复制标题
DOI：
10.3892/ijo_00000233
复制
发表时间：
2009-04-01
影响因子：
5.2
通讯作者：
Mori, Masaki
中科院分区：
文献类型：
--
作者：
Motoyama, Kazuo;Inoue, Hiroshi;Mori, Masaki
MicroRNA（miRNAs）是一类小分子（21-23个核苷酸）非编码RNA，具有转录后基因调控功能。越来越清楚的是，改变的miRNA表达与癌症的发病机制相关。本研究的目的是确定在人类结直肠癌中上调的miRNAs。从手术切除的结直肠癌的癌组织和相应的非癌组织中分离总RNA。使用含有455个人miRNA探针的miRNA微阵列测定miRNA的表达谱。然后通过实时RT-PCR研究配对临床样本中选定的miRNA的表达状态。通过miRNA阵列分析鉴定出21种miRNA在结直肠癌组织中与正常上皮组织相比过表达。其中，miR-31、miR-183、miR-17- 5 p、miR-18 a、miR-20 a和miR-92在癌组织中的表达明显高于正常组织（P
MicroRNAs (miRNAs) constitute a class of small (21-23 nucleotides) noncoding RNAs that function as post-transcriptional gene regulators. It is becoming increasingly clear that altered miRNA expression correlates with the pathogenesis of cancers. The purpose of this study was to determine the up-regulated miRNAs in human colorectal cancer. Total RNA was isolated from cancer tissues and corresponding noncancerous tissues from surgically resected colorectal cancers. The expression profiles of miRNAs were determined using a miRNA microarray containing 455 human miRNA probes. The expression status of selected miRNAs in paired clinical samples was then investigated by real-time RT-PCR. Twenty-one miRNAs were identified by miRNA array analysis as overexpressed in colorectal cancer tissues compared to normal epithelial tissues. Among them, the expression of miR-31, miR-183, miR-17-5p, miR-18a, miR-20a and miR-92 were confirmed to be significantly higher in cancer tissues than in normal tissues (P