Analysis of the tumor-associated antigen TSP-180. Identity with alpha 6-beta 4 in the integrin superfamily.
Analysis of the tumor-associated antigen TSP-180. Identity with alpha 6-beta 4 in the integrin superfamily.
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发表时间：
1989-09
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通讯作者：
S. Kennel;L. Foote;R. Falcioni;A. Sonnenberg;C. Stringer;C. Crouse;M. Hemler
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作者：
S. Kennel;L. Foote;R. Falcioni;A. Sonnenberg;C. Stringer;C. Crouse;M. Hemler
肿瘤相关抗原复合物TSP-180以前在癌细胞系中被定义，并且发现在肿瘤中的表达量高于正常组织。在此，小鼠TSP-180复合物显示由三种相关蛋白质（条带1、2和3）组成，这三种蛋白质与可能源自前体蛋白质（条带4）的不同蛋白质（条带5）相关。所有这些蛋白质都是细胞表面糖蛋白，最大的蛋白质（条带1）可以很容易地用32 PO 4标记。这里描述的小鼠TSP-180复合物与最近描述的人整合素α 6-β 4复合物非常相似。使用两种不同的大鼠抗α 6单克隆抗体证实了这种同源性，每种抗体均识别人α 6-β 4和小鼠TSP-180复合物。此外，TSP-180条带5蛋白（小鼠α 6）具有与人α 6相同的N-末端序列。最后，描述了两种不同的单克隆抗体，346- 11 A和439- 9 B，它们分别直接识别多种形式的小鼠和人β 4蛋白。
The tumor-associated antigen complex, TSP-180, was previously defined in carcinoma cell lines and found to be expressed in higher amounts in tumor than in normal tissue. Here, the mouse TSP-180 complex is shown to consist of three related proteins (bands 1, 2, and 3) associated with a distinct protein (band 5) that is probably derived from a precursor protein (band 4). All of these proteins are cell surface glycoproteins, and the largest protein (band 1) can be readily labeled with 32PO4. The mouse TSP-180 complex described here strongly resembles the recently described human integrin alpha 6-beta 4 complex. This homology was confirmed using two distinct rat anti-alpha 6 monoclonal antibodies, each of which recognized both human alpha 6-beta 4 and mouse TSP-180 complexes. Furthermore, the TSP-180 band 5 protein (mouse alpha 6) had an N-terminal sequence identical to that of human alpha 6. Finally, two different monoclonal antibodies are described, 346-11A and 439-9B, which directly recognize the multiple forms of mouse and human beta 4 proteins, respectively.