THE ISOLATION AND PARTIAL CHARACTERIZATION OF A HIGHLY PATHOGENIC HERPESVIRUS FROM THE HARBOR SEAL (PHOCA-VITULINA)
THE ISOLATION AND PARTIAL CHARACTERIZATION OF A HIGHLY PATHOGENIC HERPESVIRUS FROM THE HARBOR SEAL (PHOCA-VITULINA)
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DOI：
10.1007/bf01309828
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
VANSTEENIS, G
中科院分区：
文献类型：
--
作者：
OSTERHAUS, ADME;YANG, H;VANSTEENIS, G
本报告描述了从斑海豹（Phoca vitulina）的疱疹病毒的第一次分离和部分特征。该病毒是在荷兰一家海豹孤儿院护理的一群年轻海豹的疾病爆发期间分离出来的。几乎一半的海豹死于急性肺炎和局灶性肝炎，病毒是从大多数死亡动物的器官中分离出来的。在这次疫情期间，获得配对血清样本的10只海豹中有7只在病毒中和试验中显示血清转化。根据其在电子显微镜下的特征形态、在蔗糖中的浮力密度、对乙醚和热处理的敏感性以及与α疱疹病毒亚科其他可能成员的抗原关系，该病毒被初步鉴定为疱疹病毒（海豹疱疹病毒：SeHV或phocid疱疹病毒1）。该病毒在接种后24小时内在海豹肾细胞中引起细胞病变变化，包括细胞的局灶性变圆和合胞体形成。在其他9种受试哺乳动物种属的细胞中未观察到细胞病变。
This report describes the first isolation and partial characterization of a herpesvirus from the harbor seal (Phoca vitulina). The virus was isolated during a disease outbreak in a group of young seals nursed in an seal orphanage in The Netherlands. Almost half of the seals died with symptoms of acute pneumonia and focal hepatitis and the virus was isolated of organs of most of the dead animals. Seven out of ten seals of which paired serum samples were obtained showed seroconversion in a virus neutralization test during this outbreak. The virus was tentatively characterized as a herpes-virus (seal herpesvirus: SeHV or phocid herpesvirus 1) on the basis of its characteristic morphology in electron microscopy, buoyant density in sucrose, sensitivity to ether and heat treatment and its antigenic relationship with other probable members of the Alphaherpesvirinae subfamily. The virus caused cytopathic changes within 24 hours after inoculation in seal kidney cells, consisting of a focal rounding of cells and syncytium formation. No cytopathic changes were observed in the cells of nine other mammalian species tested.