5alpha-Androstane-3alpha,17beta-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation.
5alpha-Androstane-3alpha,17beta-diol activates pathway that resembles the epidermal growth factor responsive pathways in stimulating human prostate cancer LNCaP cell proliferation.
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5alpha-Androstane-3alpha,17beta-diol 激活类似于表皮生长因子响应途径的途径，刺激人前列腺癌 LNCaP 细胞增殖。
DOI：
10.1038/sj.pcan.4500761
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Lin,H-K
中科院分区：
文献类型：
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作者：
Zimmerman,RA;Dozmorov,I;Nunlist,EH;Tang,Y;Li,X;Cowan,R;Centola,M;Frank,MB;Culkin,DJ;Lin,H-K
5α-Androstane-3α，17β-二醇（3α-diol）被认为对雄激素靶器官没有雄激素作用，除非它被氧化成5α-二氢睾酮（5α-DHT）。我们使用微阵列和生物信息学来识别和比较人前列腺LNCaP细胞中的3α-二醇和5α-DHT反应基因。通过称为“高变量测定”的程序，在 5α-DHT、3α-二醇和表皮生长因子 (EGF) 处理的样品中选择了一组类似的 30 个反应基因，涉及信号转导、转录调控和细胞增殖。 F-均值聚类和网络程序表明，这些基因的响应模式在 3α-二醇和 EGF 之间的相关性比 5α-DHT 和 3α-二醇处理之间的相关性更密切。我们得出结论，3α-二醇能够通过引发 EGF 样途径与雄激素受体途径结合来刺激前列腺细胞增殖。
5α-Androstane-3α, 17β-diol (3α-diol) is considered to have no androgenic effects in androgen target organs unless it is oxidized to 5α-dihydrotestosterone (5α-DHT). We used microarray and bioinformatics to identify and compare 3α-diol and 5α-DHT responsive gene in human prostate LNCaP cells. Through a procedure called ‘hypervariable determination’, a similar set of 30 responsive genes involving signal transduction, transcription regulation, and cell proliferation were selected in 5α-DHT-, 3α-diol-, and epidermal growth factor (EGF)-treated samples. F-means cluster and networking procedures showed that the responsive pattern of these genes was more closely related between 3α-diol and EGF than between 5α-DHT and 3α-diol treatments. We conclude that 3α-diol is capable of stimulating prostate cell proliferation by eliciting EGF-like pathway in conjunction with androgen receptor pathway.