Comparative Immunophenotyping of Equine Multipotent Mesenchymal Stromal Cells: An Approach Toward a Standardized Definition
Comparative Immunophenotyping of Equine Multipotent Mesenchymal Stromal Cells: An Approach Toward a Standardized Definition
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DOI：
10.1002/cyto.a.22491
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发表时间：
2014-08-01
期刊：
影响因子：
3.7
通讯作者：
Burk, Janina
中科院分区：
文献类型：
--
作者：
Paebst, Felicitas;Piehler, Daniel;Burk, Janina
马是批准用于肌肉骨骼系统治疗的大型动物模型。特别是对于肌腱疾病，其中基于细胞的治疗通常用于马患者，将所取得的结果转化为人类医学将是一项伟大的成就。马间充质基质细胞（MSCs）的免疫表型仍然是最后的障碍，以满足国际细胞治疗学会（ISCT）的标准定义的人MSCs。因此，使用流式细胞术分析了来自脂肪组织、骨髓、脐带血、脐带组织和肌腱组织的马MSC中CD 29、CD 44、CD 73、CD 90、CD 105、CD 14、CD 34、CD 45、CD 79 α和MHC II的表面抗原表达。来自不同来源和供体的分离的细胞在其MSC定义抗原的表达模式上不同。特别是CD 90和105显示出最大的异质性。然而，来自所有样品的细胞对于CD 29和CD 44均为强阳性，而对于CD 73和排除标志物CD 14、CD 34、CD 45、CD 79a和MHC II大部分为阴性。此外，很明显，体外培养后用于细胞分离的酶影响抗原表达的检测。这些结果强调了MSC分离、培养和收获技术标准化的必要性。由于马MSC不符合ISCT为人MSC定义的所有标准，因此应进行进一步研究以更好地表征细胞类型。(C)2014年国际细胞计数促进学会
Horses are an approved large animal model for therapies of the musculoskeletal system. Especially for tendon disease where cell-based therapy is commonly used in equine patients, the translation of achieved results to human medicine would be a great accomplishment. Immunophenotyping of equine mesenchymal stromal cells (MSCs) remains the last obstacle to meet the criteria of the International Society for Cellular Therapy (ISCT) definition of human MSCs. Therefore, the surface antigen expression of CD 29, CD 44, CD 73, CD 90, CD 105, CD 14, CD 34, CD 45, CD 79 alpha, and MHC II in equine MSCs from adipose tissue, bone marrow, umbilical cord blood, umbilical cord tissue, and tendon tissue was analyzed using flow cytometry. Isolated cells from the different sources and donors varied in their expression pattern of MSC-defining antigens. In particular, CD 90 and 105 showed most heterogeneity. However, cells from all samples were robustly positive for CD 29 and CD 44, while being mostly negative for CD 73 and the exclusion markers CD 14, CD 34, CD 45, CD 79a and MHC II. Furthermore, it was evident that enzymes used for cell detachment after in vitro-culture affected the detection of antigen expression. These results emphasize the need of standardization of MSC isolation, culturing, and harvesting techniques. As the equine MSCs did not meet all criteria the ISCT defined for human MSCs, further investigations for a better characterization of the cell type should be conducted. (C) 2014 International Society for Advancement of Cytometry