ILV methyl NMR resonance assignments of the 81 kDa E. coli β-clamp.
ILV methyl NMR resonance assignments of the 81 kDa E. coli β-clamp.
复制标题
81 kDa 大肠杆菌 β 钳的 ILV 甲基 NMR 共振分配。
DOI：
10.1007/s12104-022-10097-0
复制
发表时间：
2022
影响因子：
0.9
通讯作者：
Korzhnev,DmitryM
中科院分区：
文献类型：
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作者：
Lim,Socheata;Mahdi,Sam;Beuning,PennyJ;Korzhnev,DmitryM
环形E.大肠杆菌β-clamp蛋白是一个81 kDa的头-尾同源二聚体，它作为一个持续合成因子将复制聚合酶锚定在DNA上，从而增加复制持续合成能力和速度。此外，它还促进了DNA在复制、修复和损伤反应过程中发生的许多蛋白质交易。我们使用基于结构的方法，基于定点突变和甲基-甲基NOESY数据分析，获得野生型β-clamp及其稳定的T45 R/S107 R变体的几乎完整的Ile、Leu和瓦尔侧链甲基NMR共振归属。所得结果将有助于进一步研究β-钳相互作用和动力学。
The ring-shapedE. coliβ-clamp protein is an 81 kDa head-to-tail homodimer, which serves as a processivity factor anchoring the replicative polymerase to DNA, thereby increasing replication processivity and speed. In addition, it facilitates numerous protein transactions that take place on DNA during replication, repair, and damage response. We used a structure-based approach to obtain nearly complete Ile, Leu and Val side-chain methyl NMR resonance assignments of the wild-type β-clamp and its stabilized T45R/S107R variant based on site directed mutagenesis and the analysis of methyl-methyl NOESY data. The obtained assignments will facilitate future studies of the β-clamp interactions and dynamics.