Functional expression of p64, an intracellular chloride channel protein.
Functional expression of p64, an intracellular chloride channel protein.
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p64（一种细胞内氯离子通道蛋白）的功能表达。
DOI：
10.1007/s002329900376
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Schlesinger,PH
中科院分区：
文献类型：
--
作者：
Edwards,JC;Tulk,B;Schlesinger,PH
p64 is a protein identified as a chloride channel by biochemical purification from kidney microsomes. We expressed p64 in HeLa cells using a recombinant vaccinia virus/T7 RNA polymerase driven system. Total cell membranes were prepared from infected/transfected cells and fused to a planar lipid bilayer. A novel chloride channel activity was found in cells expressing p64 and not in control cells. The p64-associated activity shows strong anion over cation selectivity. Single channels show prominent outward rectification with single channel conductance at positive potentials of 42 pS. The chloride channel activity is activated by treatment of the membranes with alkaline phosphatase and inhibited by DNDS and by TS-TM calix(4)arene. Whole membrane anion permeability was determined by a chloride efflux assay, revealing that membranes from cells expressing p64 showed a small but highly significant increase in chloride permeability, consistent with expression of a novel chloride channel activity.
