Reversal of blue light-stimulated stomatal opening by green light
Reversal of blue light-stimulated stomatal opening by green light
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DOI：
10.1093/pcp/41.2.171
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发表时间：
2000-02-01
影响因子：
4.9
通讯作者：
Zeiger, E
中科院分区：
文献类型：
--
作者：
Frechilla, S;Talbott, LD;Zeiger, E
绿光刺激下蚕豆离体表皮气孔打开被逆转。30秒的绿光脉冲消除了由紧接在前面的蓝光脉冲刺激的瞬态开口。随后的蓝光脉冲恢复了开口。最初的绿光脉冲不会改变对随后的蓝光脉冲的响应。在有或没有饱和红光背景的连续照明下也会发生反转。绿光反转的幅度取决于通量，在绿光的通量是蓝光的两倍时观察到完全反转。连续绿光单独照射对气孔打开有轻微的刺激作用，而对红光刺激的气孔打开没有影响。绿光效应的作用谱在540 nm处有最大值，在490 nm和580 nm处有小峰。该光谱与蓝光刺激下气孔开启的作用光谱相似，红移约90 nm。类胡萝卜素玉米黄质被认为是气孔蓝光响应的光感受器。蓝/绿的可逆性可以用一对相互转换的玉米黄质异构体来解释，一个吸收蓝色，另一个吸收绿色，绿色的吸收形式是生理上活跃的。
Blue light-stimulated stomatal opening in detached epidermis of Vicia faba is reversed by green light. A 30 s green light pulse eliminated the transient opening stimulated by an immediately preceding blue light pulse. Opening was restored by a subsequent blue light pulse. An initial green light pulse did not alter the response to a subsequent blue light pulse. Reversal also occurred under continuous illumination, with or without a saturating red light background. The magnitude of the green light reversal depended on fluence rate, with full reversal observed at a green light fluence rate twice that of the blue light. Continuous green light given alone stimulated a slight stomatal opening, and had no effect on red light-stimulated opening. An action spectrum for the green light effect showed a maximum at 540 nm and minor peaks at 490 and 580 nm. This spectrum is similar to the action spectrum for blue light-stimulated stomatal opening, red-shifted by about 90 nm. The carotenoid zeaxanthin has been implicated as a photoreceptor for the stomatal blue light response. Blue/green reversibility might be explained by a pair of interconvertible zeaxanthin isomers, one absorbing in the blue and the other in the green, with the green absorbing form being the physiologically active one.